Facile synthesis of a biocompatible silver nanoparticle derived tripeptide supramolecular hydrogel for antibacterial wound dressings. (4th January 2016)

• Record Type: Journal Article
• Authors: Simon, Turibius; Wu, Chung-Shu; Liang, Jie-Chuan; Cheng, Chieh; Ko, Fu-Hsiang
• Is Part Of: New journal of chemistry. Volume 40:Number 3(2016:Mar.)
• Page Start: 2036
• Page End: 2043
• Publication Date: 2016-01-04
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c5nj01981h
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : Through a mineralization process, Nap-FFC peptides produced transparent silver nanoparticle-based hydrogels (AgNPs@Nap-FFC) for antibacterial wound dressing. Abstract : Realizing the widespread demand for biocompatible supramolecular hydrogel based wound dressings, we have developed an N-terminally 2(naphthalen-6-yl)acetic acid (Nap) protected Phe–Phe–Cys peptide (Nap-FFC) using a liquid phase method. This Nap-FFC peptide was used to design a supramolecular hydrogel via a self-assembling process. The Nap-FFC short peptides produced stable and transparent silver nanoparticle-based hydrogels (AgNPs@Nap-FFC) wherein the self-assembled Nap-FFC nanofibers acted as scaffolds for the mineralization of silver nanoparticles (AgNPs) and stabilizers of the synthesized AgNPs. The resultant AgNPs@Nap-FFC nanocomposites were characterized using ultraviolet-visible spectrophotometry (UV-vis spectroscopy), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM) and scanning electron microscopy (SEM) combined with energy-dispersive X-ray spectroscopy (EDX) studies. The AgNPs@Nap-FFC nanocomposites showed excellent monodispersity, long term stability, and functional flexibility in comparison to other AgNP based nanocomposites. Furthermore, AgNPs@Nap-FFC exhibited strong inhibition against both Gram-positive (methicillin-resistant Staphylococcus aureus ) and Gram-negative ( Acinetobacter baumannii ) bacteria and, most importantly, they showed favorable biocompatibility towards human cervical carcinoma cells (HeLa cells). Hence, this study implies that AgNPs@Nap-FFC nanocomposites can easily be prepared in a cost-effective manner and can be used effectively for future antibacterial wound dressings.