Bacteriophage conjugated IRMOF-3 as a novel opto-sensor for S. arlettae. (10th August 2016)

• Record Type: Journal Article
• Authors: Bhardwaj, Neha; Bhardwaj, Sanjeev K.; Mehta, Jyotsana; Nayak, Manoj K.; Deep, Akash
• Is Part Of: New journal of chemistry. Volume 40:Number 9(2016:Sep.)
• Page Start: 8068
• Page End: 8073
• Publication Date: 2016-08-10
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c6nj00899b
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : This article reports the novel assembly of a bacteriophage-based fluorescent sensor for the selective and sensitive detection of a model bacterium ' Staphylococcus arlettae ( S. arlettae )'. Abstract : This article reports the novel assembly of a bacteriophage-based fluorescent sensor for the selective and sensitive detection of a model bacterium ' Staphylococcus arlettae ( S. arlettae )'. A host specific bacteriophage was bioconjugated with a fluorescence metal organic framework 'IRMOF-3'. Changes in the photoluminescence intensities of this fluorescent probe were correlated with bacterial concentrations. The proposed bacteriophage based opto-sensor provided a low detection limit (100 cfu mL −1 ) along with specificity in the detection with respect to other some non-specific bacteria, e.g. S. aureus and E. coli . The detection was achieved over a wide range of bacterial concentrations, i.e. 10 2 –10 10 cfu mL −1 S. arlettae . Compared to antibody and DNA based optical sensors, the use of bacteriophage in conjugation with IRMOF-3 should offer advantages of simplicity and stability. The use of IRMOF-3 as a fluorescent molecule should also offer the development of reproducible sensors because of its well defined structural geometry and hierarchical assembly.