A member of the immunoglobulin superfamily, orange-spotted grouper novel immune gene EcVig, is induced by immune stimulants and type I interferon. (November 2016)

• Record Type: Journal Article
• Authors: Yeh, Ying-Chun; Wang, Ting-Yu; Chou, Hsin-Yiu; Lin, Han-You; Chen, Tzong-Yueh; Aoki, Takashi; Wang, Han-Ching
• Is Part Of: Fish & shellfish immunology. Volume 58(2016:Nov.)
• Page Start: 415
• Page End: 422
• Publication Date: 2016-11
• Subjects: VHSV induced gene (Vig) -- Orange-spotted grouper (Epinephelus coioides) -- LPS -- Poly(I:C) -- Red sea bream iridovirus (RSIV) -- Grouper iridovirus (GIV); Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.09.039
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: A novel grouper immune gene, EcVig was identified in orange-spotted grouper ( Epinephelus coioides ). We recently determined that EcVig expression can be induced by infection with nervous necrosis virus (NNV, an RNA virus), whereas NNV replication may be suppressed when EcVig was overexpressed. Although EcVig appeared to be involved in grouper antiviral activity, its immune effects have not been well characterized. In the present study, two PAMPs (pathogen-associated molecular patterns; lipopolysaccharides [LPS] and synthetic double-stranded RNA polyriboinosinic-polyribocytidylic acid [poly(I:C)]), as well as fish DNA virus (red sea bream iridovirus, RSIV; grouper iridovirus, GIV), were used to study EcVig responses in orange-spotted grouper. In addition, groupers were given recombinant type I interferon to determine whether EcVig expression was induced. Poly(I:C) rapidly induced substantial expression of EcVig, whereas LPS stimulation did not appear to have any effect in grouper intestine. Expression levels of total EcVig and other IFN-stimulated genes (ISGs) were all significantly increased after RSIV and GIV infection. Furthermore, stimulation of recombinant type I IFN also increased EcVig expression. We conclude that EcVig may be a novel IFN-stimulated gene that demonstrates an antiviral immune response. Highlights: The expression level of EcVig showed no significant up-regulation after LPS stimulation. The expression level of EcVig showed significant up-regulation after poly (I:C) stimulation. The expression level of EcVig was significantly increased after DNA virus infection. The expression level of EcVig was significantly increased after rIFN stimulation. EcVig may be a novel IFN-stimulated gene.