Triggering of apoptosis in osteosarcoma cells by graphene/single‐walled carbon nanotube hybrids via the ROS‐mediated mitochondrial pathway. Issue 2 (21st October 2016)

• Record Type: Journal Article
• Authors: Yan, Xinxin; Yang, Wen; Shao, Zengwu; Yang, Shuhua; Liu, Xianzhe
• Is Part Of: Journal of biomedical materials research. Volume 105:Issue 2(2017)
• Page Start: 443
• Page End: 453
• Publication Date: 2016-10-21
• Subjects: carbon‐based nanomaterials -- osteosarcoma -- apoptosis -- 3D structure -- cytotoxicity; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.35918
• Languages: English
• ISSNs: 1549-3296

Abstract

Abstract: Carbon nanomaterials are increasingly significant in the biological and medical fields, especially becoming promising candidates in treating difficult and complicated disease. Graphene/single‐walled carbon nanotubes (G/SWCNT) hybrids is 3D structure which has been constructed by combining 1D single‐walled carbon nanotubes (SWCNTs) and 2D graphene. However, the effects of the nanomaterial on biological systems are limited. In this study, we report a systematic investigation of the cytotoxicity and in vivo biodistribution of G/SWCNT hybrids on osteosarcoma cells (HOS and U2OS). The CCK‐8, neutral red, and lactic dehydrogenase assays demonstrated that the cytotoxicity of G/SWCNT hybrids exhibits a dose‐dependent behavior on osteosarcoma cells. In our conditions, the hybrids were less cytotoxic than graphene and single‐walled carbon nanotubes. The results also showed the apoptosis of osteosarcoma cells induced by G/SWCNT hybrids was through the increase of intracellular reactive oxygen species, the decrease of mitochondrial membrane potential, the alternation of apoptosis‐related proteins, and then triggered the ROS‐mediated mitochondrial pathway. Moreover, the in vivo biodistribution of G/SWCNT hybrids was observed by histological analysis of major organs in mice, and showed that organs were neither damaged nor inflammatory. This study demonstrated that G/SWCNT hybrids could serve as a potential platform in anticancer therapy. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 443–453, 2017.