Characterizing polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB+1. Issue 24 (22nd November 2016)

• Record Type: Journal Article
• Authors: Chojnacki, Michal; Zhang, Daoning; Talarowska, Monika; Gałecki, Piotr; Szemraj, Janusz; Fushman, David; Nakasone, Mark A.
• Is Part Of: FEBS letters. Volume 590:Issue 24(2016)
• Page Start: 4573
• Page End: 4585
• Publication Date: 2016-11-22
• Subjects: biomolecular NMR -- deubiquitinase -- neurodegeneration -- polyubiquitin -- UBB +1; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.12484
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : The ubiquitin mutant UBB +1 has been identified as a hallmark of neurodegenerative diseases. In this study, we characterize polyubiquitinated forms of UBB +1 in vitro and from patient samples. The ability of UBB +1 to be readily ubiquitinated by several E2 enzymes provided a mechanism for the controlled synthesis and purification of defined conjugates. This allowed us to utilize polyUb–UBB +1 conjugates for biochemical assays, as well as solution NMR. Coupled with our immunoassay for detection of ubiquitinated forms of UBB +1 in patient blood samples, we gain a clearer picture of the molecular mechanisms underlying neurodegenerative diseases. Abstract :