Dabsyl chloride derivatisation of melamine followed by high-performance liquid chromatography determination in water samples. Issue 15 (7th December 2016)

• Record Type: Journal Article
• Authors: Faraji, Mohammad; Amini Kadijani, Parisa
• Is Part Of: International journal of environmental analytical chemistry. Volume 96:Issue 15(2016)
• Page Start: 1430
• Page End: 1439
• Publication Date: 2016-12-07
• Subjects: Melamine -- dabsyl chloride -- high-performance liquid chromatography -- derivatisation; Ecology -- Periodicals; Chemistry, Analytic -- Periodicals; Environmental chemistry -- Periodicals; Pollution -- Periodicals; 577.14
• DOI: 10.1080/03067319.2016.1267160
• Languages: English
• ISSNs: 0306-7319

Abstract

ABSTRACT: A new and sensitive precolumn derivatisation with dabsyl chloride was developed for the analysis of melamine in water samples by high-performance liquid chromatography (HPLC) with visible detection. Derivatisation with dabsyl chloride leads to improving sensitivity and hydrophobicity of melamine. Under optimum conditions of derivatisation and microextraction, the method yielded a linear calibration curve ranging from 10 to 2000 µg L −1 with a determination coefficient (R 2 ) of 0.9952. Limit of detection (LOD) and limit of quantification (LOQ) were 2.0 and 6.0 µg L −1, respectively. The relative standard deviation per cent (RSD%) for intraday and inter-day extraction and determination at 20 and 200 µg L −1 levels of melamine was less than 8.2% ( n = 6). Finally, the proposed method was successfully applied for the determination of melamine in different water samples and satisfactory results were obtained (relative recovery ≥91%).