Cryopreservation of strip spawned sperm using non‐programmable freezing technique in the blue mussel Mytilus galloprovincialis. (14th July 2015)

• Record Type: Journal Article
• Authors: Liu, Bingli; Liu, Yibing; Liu, Shiwen; Xu, Tong; Liu, Qing; Li, Xiaoxu
• Is Part Of: Aquaculture research. Volume 47:Number 12(2016)
• Page Start: 3888
• Page End: 3898
• Publication Date: 2015-07-14
• Subjects: cryopreservation -- blue mussel -- Mytilus galloprovincialis -- strip spawned sperm -- non‐programmable freezing technique -- glycine; Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.12839
• Languages: English
• ISSNs: 1355-557X

Abstract

Abstract: In this study, a non‐programmable freezing technique has been developed with the strip spawned blue mussel ( Mytilus galloprovincialis ) sperm. The key parameters optimized including (1) cryoprotectant agents (CPAs); (2) cooling temperature; (3) thawing temperature; (4) sugar and amino acid supplementation and (5) sperm to egg ratio. The fertilization rate and/or integrity of plasma membrane and acrosome were used as sperm quality assessment indicators. The highest post‐thaw sperm fertilization rate of 95% was achieved, when sperm were cryopreservated in 8% dimethyl sulfoxide at 7.8 cm above the liquid nitrogen surface and thawed in a 60°C seawater bath. The addition of glucose, sucrose or trehalose in dimethyl sulfoxide did not, whereas 0.8% glycine did significantly improve the post‐thaw sperm fertilization rates. The fluorescent evaluation has demonstrated that the addition of glycine significantly improved the post‐thaw sperm acrosome integrity, revealing a positive role of glycine in the improvement of post‐thaw sperm quality in blue mussels.