Embryonic atrazine exposure alters zebrafish and human miRNAs associated with angiogenesis, cancer, and neurodevelopment. (December 2016)

• Record Type: Journal Article
• Authors: Wirbisky, Sara E.; Weber, Gregory J.; Schlotman, Kelly E.; Sepúlveda, Maria S.; Freeman, Jennifer L.
• Is Part Of: Food and chemical toxicology. Volume 98:Part A(2016)
• Page Start: 25
• Page End: 33
• Publication Date: 2016-12
• Subjects: Atrazine -- Development -- miRNA -- Targeting analysis -- Transcriptomics -- Zebrafish; ANOVA analysis of variance -- CNS central nervous system -- EDCs endocrine disrupting chemicals -- FSH follicle stimulating hormone -- GnRH gonadotropin releasing hormone -- hpf hours post fertilization -- IPA Ingenuity Pathway Analysis -- ISVs intersegmental vessels -- LH luteininzing hormone -- miRNA microRNA -- ppb parts per billion -- PRL prolactin -- US EPA United States Environmental Protection Agency; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2016.03.027
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: MicroRNAs (miRNAs) are short, single-stranded RNA that regulate post-transcriptional control of mRNA translation. Knowledge on the role of these critical regulators in toxicological responses in increasing, but is still limited. Atrazine is a herbicide used throughout the Midwestern US that is reported to frequently contaminate potable water supplies above the maximum contaminant level of 3 parts per billion. Atrazine is a suspected endocrine disrupting chemical and studies have begun to investigate the genetic mechanisms of toxicity; however, studies investigating epigenetic mechanisms are limited. In this study both zebrafish and human miRNAs were significantly altered in response to an embryonic atrazine exposure of 0.3, 3, or 30 ppb in zebrafish. Altered miRNAs are known to play a role in angiogenesis, cancer, or neuronal development, differentiation, and maturation. Targeted analysis of altered human miRNAs with genes previously identified to be altered by atrazine exposure revealed several targets linked to cell cycle and cell signaling. Further analysis of hsa-miRNA-126-3p, which had altered expression in all three atrazine treatments at 72 hpf, revealed alterations also occurred at 60 hpf in the 30 ppb treatment group. Results from this study indicate miRNA deregulation in zebrafish and human miRNAs following an embryonic atrazine exposure in zebrafish. Highlights: Developmental atrazine exposure alters zebrafish and human miRNAs. Targeted analysis links miRNAs to mRNA involved in cell signaling and cell cycle. hsa-miR-126-3p was characterized through development and following developmental atrazine exposure.