Taiwanin C selectively inhibits arecoline and 4‐NQO‐induced oral cancer cell proliferation via ERK1/2 inactivation. Issue 1 (5th November 2015)

• Record Type: Journal Article
• Authors: Lin, Kuan‐Ho; Shibu, Marthandam Asokan; Kuo, Yueh‐Hsiung; Chen, Yueh‐Chiu; Hsu, Hsi‐Hsien; Bau, Da‐Tian; Chen, Ming‐Cheng; Tu, Chuan‐Chou; Viswanadha, Vijaya Padma; Huang, Chih‐Yang
• Is Part Of: Environmental toxicology. Volume 32:Issue 1(2017)
• Page Start: 62
• Page End: 69
• Publication Date: 2015-11-05
• Subjects: arecoline -- oral cancer -- taiwanin C -- Taiwania cryptomerioides; Water quality bioassay -- Periodicals; Water -- Pollution -- Toxicology -- Periodicals; Microbiological assay -- Periodicals; Toxicity testing -- Periodicals; Environmental toxicology -- Periodicals; Environmental Pollution -- Periodicals; Environmental Pollutants -- Periodicals; Environmental Monitoring -- Periodicals; Écotoxicologie -- Périodiques; Pollution -- Périodiques; 615.902
• DOI: 10.1002/tox.22212
• Languages: English
• ISSNs: 1520-4081

Abstract

ABSTRACT: Arecoline, the most abundant alkaloid in betel nut is known to promote abnormal proliferation of epithelial cells by enhancing epidermal growth factor receptor (EGFR) activation and cyclooxygenase‐2 (COX2) expression. Taiwanin C, a naturally occurring lignan extracted from Taiwania cryptomerioides, has been found to be a potential inhibitor of COX2 expression. Based on the MTT assay results, taiwanin C was found to be effective in inhibiting the tumorous T28 cell than the non‐tumorous N28 cells. The modulations in the expression of relevant proteins were determined to understand the mechanism induced by taiwanin C to inhibit T28 cell proliferation. The levels of activated EGFR and COX2 were found to be abnormally high in the T28 oral cancer cells. However, taiwanin C was found to inhibit the activation of EGFR and regulated other related downstream proteins and thereby inhibited the T28 cell proliferation. In conclusion the results indicate that taiwanin C suppresses COX2‐EGFR and enhances P27 pathways to suppress arecoline induced oral cancer cell proliferation via ERK1/2 inactivation. © 2015 Wiley Periodicals, Inc. Environ Toxicol 32: 62–69, 2017.