Cold atmospheric plasma treatment of melanoma and glioblastoma cancer cells. Issue 12 (31st October 2016)

• Record Type: Journal Article
• Authors: Vermeylen, Stijn; De Waele, Jorrit; Vanuytsel, Steven; De Backer, Joey; Van der Paal, Jonas; Ramakers, Marleen; Leyssens, Karen; Marcq, Elly; Van Audenaerde, Jonas; L. J. Smits, Evelien; Dewilde, Sylvia; Bogaerts, Annemie
• Other Names: Laroussi Mounir guestEditor.; Bogaerts Annemie guestEditor.; Barekzi Nazir guestEditor.
• Is Part Of: Plasma processes and polymers. Volume 13:Issue 12(2016)
• Page Start: 1195
• Page End: 1205
• Publication Date: 2016-10-31
• Subjects: cancer cell -- viability -- experimental parameters -- glioblastoma -- melanoma -- non‐thermal plasma; Plasma polymerization -- Periodicals; Plasma-enhanced chemical vapor deposition -- Periodicals; Plasma chemistry -- Periodicals
• DOI: 10.1002/ppap.201600116
• Languages: English
• ISSNs: 1612-8850

Abstract

Abstract : In this paper, two types of melanoma and glioblastoma cancer cell lines are treated with cold atmospheric plasma to assess the effect of several parameters on the cell viability. The cell viability decreases with treatment duration and time until analysis in all cell lines with varying sensitivity. The majority of dead cells stains both AnnexinV (AnnV) and propidium iodide, indicating that the plasma‐treated non‐viable cells are mostly late apoptotic or necrotic. Genetic mutations might be involved in the response to plasma. Comparing the effects of two gas mixtures, as well as indirect plasma‐activated medium versus direct treatment, gives different results per cell line. In conclusion, this study confirms the potential of plasma for cancer therapy and emphasizes the influence of experimental parameters on therapeutic outcome. Abstract : Apoptotic or necrotic cell death of two types of skin and brain cancer cell lines are induced by direct atmospheric plasma treatment as well as the indirect method of plasma‐activated medium. Genetic mutations might play a role in sensitivity to plasma therapy. Experimental and analysis parameters are both dictating factors in the resulting therapeutic outcome.