A Self‐Immobilizing and Fluorogenic Probe for β‐Lactamase Detection. Issue 24 (16th November 2016)

• Record Type: Journal Article
• Authors: Mao, Wuyu; Xia, Lingying; Wang, Yaqun; Xie, Hexin
• Is Part Of: Chemistry, an Asian journal. Volume 11:Issue 24(2016)
• Page Start: 3493
• Page End: 3497
• Publication Date: 2016-11-16
• Subjects: antibiotic resistance -- enzymes -- fluorescent probes -- immobilization -- β-lactamase; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/asia.201601344
• Languages: English
• ISSNs: 1861-4728

Abstract

Abstract: The spread of antibiotic resistance in pathogenic bacteria has become one of the major concerns to public health. Improved monitoring of drug resistance is of high importance for infectious disease control. One of the major mechanisms for bacteria to overcome treatment of antibiotics is the production of β‐lactamases, which are enzymes that hydrolyze the β‐lactam ring of the antibiotic. In this study, we have developed a self‐immobilizing and fluorogenic probe for the detection of β‐lactamase activity. This fluorogenic reagent, upon activation by β‐lactamases, turns on a fluorescence signal and, more importantly, generates a covalent linkage to the target enzymes or the nearby proteins. The covalent labeling of enzymes was confirmed by SDS‐PAGE analysis and MALDI‐TOF mass spectrometry. The utility of this structurally simple probe was further confirmed by the fluorescent labeling of a range of β‐lactamase‐expressing bacteria. Abstract : Bacteria are not irresistible : A self‐immobilizing and fluorogenic probe for the detection of β‐lactamase activity has been developed. This fluorogenic reagent, upon activation by β‐lactamases, turns on a fluorescence signal and, more importantly, generates a covalent linkage to the target enzymes or the nearby proteins.