Novel naproxen-peptide-conjugated amphiphilic dendrimer self-assembly micelles for targeting drug delivery to osteosarcoma cells. Issue 65 (22nd June 2016)

• Record Type: Journal Article
• Authors: Zhao, Yinbo; Zeng, Qi; Wu, Fengbo; Li, Jing; Pan, Zhaoping; Shen, Pengfei; Yang, Lu; Xu, Ting; Cai, Lulu; Guo, Li
• Is Part Of: RSC advances. Volume 6:Issue 65(2016)
• Page Start: 60327
• Page End: 60335
• Publication Date: 2016-06-22
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra15022e
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The aim of the current study was to synthesize and prepare novel self-assembly micelles loaded with curcumin (Cur) based on naproxen (Nap)-conjugated amphiphilic peptide dendrimers. Abstract : The aim of the current study was to synthesize and prepare novel self-assembly micelles loaded with curcumin (Cur) based on naproxen (Nap)-conjugated amphiphilic dendrimers. The apoptosis-inducing capacity of Nap-conjugated dendrimers and curcumin, the efficiency of uptake and the potential molecular mechanism on human osteosarcoma cells were investigated. The Nap-conjugated amphiphilic dendrimers were successfully synthesized, and the corresponding Cur-loaded micelles were conventionally prepared via self-assembly. These micelles showed good drug-encapsulating capacity, physiochemical properties, and drug-release profiles. The cellular proliferation and uptake assay suggested that the Cur-M-Nap induced more apoptosis of MG-63 human osteosarcoma cells. The western blot results suggested that these Nap-modified micelles could enhance the Cur-inducing apoptosis, mainly via intrinsic pathways and inhibition of the inflammation pathway. In summary, the Cur-loaded amphiphilic dendrimer-based micelles were successfully developed and they enhanced drug delivery to MG-63 cells. Mechanistic experiments suggested that Cur loaded into amphiphilic dendrimer-based micelles had a higher proliferation-inhibiting ability than free Cur, and could induce more apoptosis.