The antioxidative effects of three lactobacilli on high-fat diet induced obese mice. Issue 70 (11th July 2016)

• Record Type: Journal Article
• Authors: Song, Wei; Song, Chen; Shan, Yujuan; Lu, Weihong; Zhang, Jiliang; Hu, Panpan; Wu, Xianfan; Li, L.; Guo, Shouli
• Is Part Of: RSC advances. Volume 6:Issue 70(2016)
• Page Start: 65808
• Page End: 65815
• Publication Date: 2016-07-11
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra06389f
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : In this paper, three Lactobacillus strains ( L. coryniformis subsp. torquens T3, L. paracasei subsp. paracasei M5 and L. paracasei subsp. paracasei X12) isolated in our laboratory were investigated for antioxidant activity in vitro and in vivo . Abstract : In this paper, three Lactobacillus strains ( L. coryniformis subsp. torquens T3, L. paracasei subsp. paracasei M5 and L. paracasei subsp. paracasei X12) isolated in our laboratory were investigated for antioxidant activity in vitro and in vivo . Hydroxyl and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities and reducing power were examined in vitro . Three Lactobacillus strains were administered to high-fat diet induced obese mice. The results indicated that T3 and M5 showed the highest hydroxyl radical and DPPH scavenging activities at a dose of 10 9 CFU mL −1, with inhibition rates of 40.33, 41.11% and 40.76, 44.31%, respectively. The reducing activities of these two strains were also significantly higher than those of X12. Compared with the high-fat diet group, M5 and T3 have effectively controlled body weight and hepatomegaly and significantly decreased blood triglyceride and total cholesterol levels ( p < 0.05). Besides, M5 and T3 obviously improved the antioxidative indexes including MDA levels, T-SOD, GSH-Px, CAT and T-AOC activities. Overall, M5 and T3 have a strong antioxidant activity in vitro and can improve oxidative damage and control body weight in mice, suggesting that they may be potential anti-obesity and antioxidant probiotics.