Mapping of methionine-enkephalin-arg6-gly7-leu8 in the human diencephalon. (15th October 2016)

• Record Type: Journal Article
• Authors: Sánchez, M.L.; Díaz-Cabiale, Z.; Narváez, J.A.; Manso, B.; Salinas, P.; Rivada, E.; Smith, V.; Coveñas, R.
• Is Part Of: Neuroscience. Volume 334(2016)
• Page Start: 245
• Page End: 258
• Publication Date: 2016-10-15
• Subjects: 3V third ventricle -- ac anterior commissure -- ACTH adrenocorticotropin hormone -- AD anterodorsal thalamic nucleus -- al ansa lenticularis -- AM anteromedial thalamic nucleus -- Apr anteroprincipal thalamic nucleus -- bic brachium of the inferior colliculus -- BSTC bed nucleus of the stria terminalis central division -- BSTL bed nucleus of the stria terminalis lateral division -- BSTP bed nucleus of the stria terminalis posterior part -- CM centromedial thalamic nucleus -- cp cerebral peduncle -- DHA dorsal hypothalamic area -- DMH dorsomedial hypothalamic nucleus -- eml external medullary lamina of the thalamus -- Fa fasciculosus nucleus -- fx fornix -- H1 field H1(thalamic fasciculus) -- H2 fiel H2 (lenticular fasciculus) -- Hb habenula -- ic internal capsule -- IGPul intergeniculate pulvinar -- iml internal medullary lamina of the thalamus -- Inf infundibular nucleus (arcuate nucleus) -- IPul inferior pulvinar nucleus -- IthA interthalamic adhesion -- ithp inferior thalamic peduncle -- LD laterodorsal thalamic nucleus -- LDSF laterodorsal nucleus superficial part -- LG lateral geniculate nucleus -- LHA lateral hypothalamic area -- Lim limitans thalamic nucleus -- LM lateral mammillary nucleus -- LPul lateral pulvinar nucleus -- LTu lateral tuberal nucleus -- MD medial dorsal thalamic nucleus -- MDD medial dorsal thalamic nucleus dorsal part -- MDFa medial dorsal thalamic nucleus pars fasciculosa -- MDFi medial dorsal thalamic nucleus pars fibrosa -- MDMC medial dorsal thalamic nucleus pars magnocellularis -- MDV medial dorsal thalamic nucleus ventral part -- Met-8 methionine-enkephalin-Arg6-Gly7-Leu8 -- mfb medial forebrain bundle -- MGDP medial geniculate nucleus dorsoposterior part -- MGFi medial geniculate nucleus fibrosus part -- MGLi medial geniculate nucleus limitans part -- MMC medial mammillary nucleus magnocellular part -- MPC medial mammillary nucleus parvocellular part -- MPul medial pulvinar nucleus -- mt mammillo-thalamic tract -- opt optic tract -- or optic radiation -- ox optic chiasm -- Pa paraventricular hypothalamic nucleus -- PaF parafornical nucleus -- PaFo paraventricular hypothalamic nucleus fornical part -- PalHy pallidohypothalamic nucleus -- PaMc paraventricular hypothalamic nucleus magnocellular part -- PaPo paraventricular hypothalamic nucleus posterior part -- PBS phosphate-buffered saline -- pc posterior commissure -- PF parafascicular thalamic nucleus -- PHA posterior hypothalamic area -- phy pallidohypothalamic fibers (X-bundle) -- Pi pineal gland -- PT paratenial thalamic nucleus -- PV paraventricular thalamic nucleus -- Re reuniens thalamic nucleus -- Rt reticular thalamic nucleus -- SFPul superficial pulvinar nucleus -- sm stria medullaris of the thalamus -- SO supraoptic nucleus -- SODL supraoptic nucleus dorsolateral part -- SOT supraoptic nucleus tuberal part -- SOVM supraoptic nucleus ventromedial part -- sox supraoptic decussation -- st stria terminalis -- STh subthalamic nucleus -- SubH subhabenular nucleus -- TM tuberomammillary nucleus -- tt tenia thalamus -- VA ventroanterior thalamic nucleus -- VAMC ventroanterior thalamic nucleus magnocellular part -- VIM ventrointermedius nucleus -- VIME ventrointermedius nucleus external part -- VIMI ventrointermedius nucleus internal part -- VLAE ventrolateral anterior thalamic nucleus external part -- VLAI ventrolateral anterior thalamic nucleus internal part -- VLP ventrolateral posterior thalamic nucleus -- VMH ventromedial hypothalamic nucleus -- VPI ventroposterior inferior thalamic nucleus -- VPL ventral posterolateral thalamic nucleus -- VPLA ventral posterolateral thalamic nucleus anterior part -- VPM ventroposterior medial thalamic nucleus -- ZI zona incerta; met-8 -- pro-enkephalin -- thalamus -- hypothalamus -- man -- immunocytochemistry; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2016.08.010
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: The chemical neuroanatomy of the pro-enkephalin system (Met-8-immunoreactivity) is reported in the human diencephalon. Six populations of cell bodies containing Met-8 were observed. Perikarya were mainly found in the hypothalamus. The lateral hypothalamic area showed the highest density of immunoreactive cell bodies. Peptidergic fibers were more widely distributed in the hypothalamus than in the thalamus. The density of the immunoreactive fibers observed in the diencephalic nuclei was low or moderate. Abstract: Using an immunohistochemical technique, we mapped the immunoreactive structures containing methionine-enkephalin-Arg 6 -Gly 7 -Leu 8 (Met-8) (a marker for the pro-enkephalin system) in the human diencephalon. Compared with previous studies, we observed a more widespread distribution of Met-8 in the human diencephalon. Met-8-immunoreactive cell bodies and fibers exhibited a more widespread distribution in the hypothalamus than in the thalamus. We observed six populations of Met-8-immunoreactive cell bodies. These perikarya were observed in the paratenial thalamic nucleus, ventromedial and dorsomedial hypothalamic nuclei, lateral hypothalamic area, pallidohypothalamic nucleus and in the paraventricular hypothalamic nucleus (posterior part). In the thalamus, Met-8-immunoreactive fibers were primarily observed in the midline region, whereas in the hypothalamus, these fibers were widely distributed. In general, a moderate/low density of Met-8-immunoreactive fibers was observed in the diencephalic nuclei. A moderate density was observed in the paraventricular thalamic nucleus, reuniens thalamic nucleus, lateral and medial geniculate nuclei, dorsomedial hypothalamic nucleus, paraventricular hypothalamic nucleus (posterior part) and ventromedial hypothalamic nucleus. The present study is the first to demonstrate the presence of clusters of Met-8-immunoreactive cell bodies in the human thalamus and hypothalamus, the distribution of fibers containing neuropeptides in the hypothalamus and the presence of these fibers in several thalamic nuclei. This neuroanatomical study will serve to elucidate the physiological roles of Met-8 in future studies of the human diencephalon.