Maxillofacial bone regeneration with osteogenic matrix cell sheets: An experimental study in rats. (December 2016)

• Record Type: Journal Article
• Authors: Ueyama, Yoshihiro; Yagyuu, Takahiro; Maeda, Masahiko; Imada, Mitsuhiko; Akahane, Manabu; Kawate, Kenji; Tanaka, Yasuhito; Kirita, Tadaaki
• Is Part Of: Archives of oral biology. Volume 72(2016)
• Page Start: 138
• Page End: 145
• Publication Date: 2016-12
• Subjects: BMSC bone marrow-derived stromal cell -- OMCS osteogenic matrix cell sheet -- Dex dexamethasone -- H&E hematoxylin and eosin -- OPN osteopontin -- OCN osteocalcin -- micro-CT microcomputed tomography -- TCP tricalcium phosphate; Bone regeneration -- Mandibular defect -- Bone marrow-derived stromal cells -- Cell sheet; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2016.08.017
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: We previously reported the method for creating cell sheets with osteogenic ability. We make the sheets from mesodermal cells using a scraper. It was unclear whether the sheets could repair the neuroectodermal bones. Our study showed the sheets could regenerate the neuroectodermal bones. The sheets can be applied for the alveolar cleft osteoplasty. Abstract: Objective: Regeneration of maxillofacial bone defects, characterized by relatively small but complicated shapes, poses a significant clinical challenge. Osteogenic matrix cell sheets (OMCSs) have osteogenic ability and good shaping properties and may be ideal graft materials. Here, we assessed whether implantation of OMCSs could be used to repair maxillofacial bone defects. Design: We adopted a rat mandibular symphysis model. The rat mandible is formed by a paired bone and the central portion consisting of fibrous tissue. There is no bone tissue at the site; accordingly, this site was interpreted as a physiological bone gap and was used for evaluation. Rat bone marrow cells were cultured in medium containing dexamethasone and ascorbic acid phosphate to create OMCSs. The OMCSs were implanted into the rat mandibular symphysis without a scaffold. Microcomputed tomography and histological analyses were conducted after 2, 4, and 8 weeks. Results: Two weeks after implantation, microcomputed tomography images and histological sections showed some sparse granular calcification tissue within the bone gap at the mandibular symphysis. At 4 weeks, the calcification tissue spread, and the gap of the mandibles were continued. At 8 weeks, this continuous new bone tissue was matured. The experimental group showed abundant new bone tissue in the group with OMCS implantation, but not in the group with sham implantation. Conclusions: Our present results indicated that use of OMCSs may be an optimal approach towards achieving maxillofacial regeneration.