A highly sensitive fluorescent probe for the detection of bisulfite ion and its application in living cells. (January 2017)

• Record Type: Journal Article
• Authors: Yang, Yutao; Bai, Bozan; Xu, Wenzhi; Xu, Zhidong; Zhang, Jinchao; Li, Wei
• Is Part Of: Dyes and pigments. Volume 136(2017)
• Page Start: 830
• Page End: 835
• Publication Date: 2017-01
• Subjects: Direct visualisation -- Fluorescent -- Bisulfite -- Coumarin; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2016.09.047
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: A novel direct visualisation, fluorescent probe derived from coumarin for bisulfate ion detection has been designed and synthesized. This probe shows a rapid, high selectivity, and sensitivity in the detection of bisulfate ion. In a solution of DMSO:HEPES (1:9, v:v, 10 mM, pH 7.4) buffer containing the probe, the presence of HSO3 − made the UV–vis absorption of the probe blue-shift from pink to yellow, and the fluorescence of the probe also changed from red to green. Other investigated analytes and ions did not disturb the determination of HSO3 − . The reaction mechanism of the determination was proposed based on the nucleophilic attack toward electron-poor CC bond of the probe. This probe has been successfully applied for determination of HSO3 − in living cells. Graphical abstract: Highlights: A novel probe (1 ) derived from coumarin was synthesized. The probe is a sensitive fluorescent agent for the detection of bisulfite. The potential application of the probe in bioimaging was also illustrated.