Book lung development in embryos of the cobweb spider, Parasteatoda tepidariorum C. L. Koch, 1841 (Araneomorphae, Theridiidae). Issue 6 (November 2016)

• Record Type: Journal Article
• Authors: Farley, Roger D.
• Is Part Of: Arthropod structure & development. Volume 45:Issue 6(2016:Nov.)
• Page Start: 562
• Page End: 584
• Publication Date: 2016-11
• Subjects: Tissue polarity -- Book gills -- Spider development -- Lumen formation -- Secretion -- Planar cell polarity; Arthropoda -- Morphology -- Periodicals; Arthropoda -- Anatomy -- Periodicals; Arthropoda -- Cytology -- Periodicals; Arthropods -- growth & development -- Periodicals; 595
• DOI: 10.1016/j.asd.2016.09.008
• Languages: English
• ISSNs: 1467-8039

Abstract

Abstract: Light and transmission electron microscopy were used to study the development of book lungs in embryos of the spider Parasteatoda tepidariorum . There is a bilateral cluster of temporary lamellae that form just posterior to the second opisthosomal (O2) limb buds. These lamellae are replaced by advanced embryo (AE) book lungs that continue into postembryonic stages. Results herein agree with earlier suggestions that the O2 limb buds become the AE book lungs. Each O2 limb bud merges with the ventral surface of the O2 segment, where the limb bud/book lung is internalized by covering with epidermis. A strand of tissue (entapophysis) from the epidermis at the posterior opisthosoma provides precursor cells for the book lung lamellae, and possibly entapophysis cells induce limb bud cells to align and produce lamellae. Electron micrographs show the different modes (I–III) of lumen formation. The result is a spiracle, atrium and alternating air and hemolymph channels. A hypothesis is presented for the role of precursor cell polarity in producing the planar tissue polarity of the channels. Some type of apical/apical affinity results in air channels, while basal/basal affinity results in hemolymph channels. Strong basal/basal affinity is likely as opposed cells in hemolymph channels extend basal processes that span the channel and start pillar trabeculae that continue in postembryonic stages. Highlights: Temporary lamellae form just posterior to the opisthosomal second (O2) limb buds. In more advanced embryos, the O2 limb buds become continuing book lungs. A tissue strand (entapophysis) provides cells and may induce others to form lamellae. Electron micrographs show lumen formation for air and hemolymph channels and atria. A hypothesis is presented for the transition from cell polarity to lamellar tissue polarity.