Genome-scale metabolic reconstruction for the insidious bacterium in aquaculture Piscirickettsia salmonis. (January 2017)

• Record Type: Journal Article
• Authors: Fuentealba, Pablo; Aros, Camila; Latorre, Yesenia; Martínez, Irene; Marshall, Sergio; Ferrer, Pau; Albiol, Joan; Altamirano, Claudia
• Is Part Of: Bioresource technology. Volume 223(2017)
• Page Start: 105
• Page End: 114
• Publication Date: 2017-01
• Subjects: Piscirickettsia salmonis -- Genome-scale metabolic model -- Metabolic reconstruction -- Nutritional requirement -- Defined medium; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.10.024
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: First genome scale metabolic model was reconstructed for Piscirickettsia salmonis. P. salmonis catabolised amino acid as carbon and energy source. The metabolic reconstruction of P. salmonis comprising 445 reactions. The model reproduces the growth rate measure in defined liquid broth. The model is a metabolism analysis tool of P. salmonis. Abstract: Piscirickettsia salmonis is a fish bacterium that causes the disease piscirickettsiosis in salmonids. This pathology is partially controlled by vaccines. The lack of knowledge has hindered its culture on laboratory and industrial scale. The study describes the metabolic phenotype of P. salmonis in culture. This study presents the first genome-scale model ( i PF215) of the LF-89 strain of P. salmonis, describing the central metabolic pathway, biosynthesis and molecule degradation and transport mechanisms. The model was adjusted with experiment data, allowing the identification of the capacities that were not predicted by the automatic annotation of the genome sequences. The i PF215 model is comprised of 417 metabolites, 445 reactions and 215 genes, was used to reproduce the growth of P. salmonis (μmax 0.052 ± 0.005 h −1 ). The metabolic reconstruction of the P. salmonis LF-89 strain obtained in this research provides a baseline that describes the metabolic capacities of the bacterium and is the basis for developing improvements to its cultivation for vaccine formulation.