Isolation, identification and characterization of Cystobasidium oligophagum JRC1: A cellulase and lipase producing oleaginous yeast. (January 2017)

• Record Type: Journal Article
• Authors: Vyas, Sachin; Chhabra, Meenu
• Is Part Of: Bioresource technology. Volume 223(2017)
• Page Start: 250
• Page End: 258
• Publication Date: 2017-01
• Subjects: Cystobasidium oligophagum -- Rhodotorula oligophaga -- Oleaginous yeast -- Cellulase -- Cellulolytic yeast -- Lipase -- FAME; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.10.039
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Oleaginous yeast capable of cellulase and lipase production was isolated. It can grow on wide range of substrates including carboxylmethylcellulose (CMC). It can accumulate 36.46% (w/w) lipid on the medium with CMC as sole carbon source. Intracellular and extracellular lipase activity: 2.16 and 2.88 IU/mg respectively. The FAME composition suitable for use as biodiesel (glucose medium). Abstract: Oleaginous yeast closely related to Cystobasidium oligophagum was isolated from soil rich in cellulosic waste. The yeast was isolated based on its ability to accumulate intracellular lipid, grow on carboxymethylcellulose (CMC) and produce lipase. It could accumulate up to 39.44% lipid in a glucose medium (12.45 ± 0.97 g/l biomass production). It was able to grow and accumulate lipids (36.46%) in the medium containing CMC as the sole carbon source. The specific enzyme activities obtained for endoglucanase, exoglucanase, and β-glucosidase were 2.27, 1.26, and 0.98 IU/mg respectively. The specific enzyme activities obtained for intracellular and extracellular lipase were 2.16 and 2.88 IU/mg respectively. It could grow and accumulate lipids in substrates including glycerol (42.04%), starch (41.54%), xylose (36.24%), maltose (26.31%), fructose (24.29%), lactose (21.91%) and sucrose (21.72%). The lipid profile of the organism was suitable for obtaining biodiesel with desirable fuel properties.