Hyaluronic Acid/PLGA Core/Shell Fiber Matrices Loaded with EGCG Beneficial to Diabetic Wound Healing. Issue 23 (2nd November 2016)

• Record Type: Journal Article
• Authors: Shin, Yong Cheol; Shin, Dong‐Myeong; Lee, Eun Ji; Lee, Jong Ho; Kim, Ji Eun; Song, Sung Hwa; Hwang, Dae‐Youn; Lee, Jun Jae; Kim, Bongju; Lim, Dohyung; Hyon, Suong‐Hyu; Lim, Young‐Jun; Han, Dong‐Wook
• Is Part Of: Advanced healthcare materials. Volume 5:Issue 23(2016)
• Page Start: 3035
• Page End: 3045
• Publication Date: 2016-11-02
• Subjects: core/shell fiber matrix -- diabetic wound healing -- epigallocatechin‐3‐O‐gallate -- hyaluronic acid -- poly(lactic‐co‐glycolic acid); Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201600658
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract : During the last few decades, considerable research on diabetic wound healing strategies has been performed, but complete diabetic wound healing remains an unsolved problem, which constitutes an enormous biomedical burden. Herein, hyaluronic acid (HA)/poly(lactic‐ co ‐glycolic acid, PLGA) core/shell fiber matrices loaded with epigallocatechin‐3 ‐O‐ gallate (EGCG) (HA/PLGA‐E) are fabricated by coaxial electrospinning. HA/PLGA‐E core/shell fiber matrices are composed of randomly‐oriented sub‐micrometer fibers and have a 3D porous network structure. EGCG is uniformly dispersed in the shell and sustainedly released from the matrices in a stepwise manner by controlled diffusion and PLGA degradation over four weeks. EGCG does not adversely affect the thermomechanical properties of HA/PLGA‐E matrices. The number of human dermal fibroblasts attached on HA/PLGA‐E matrices is appreciably higher than that on HA/PLGA counterparts, while their proliferation is steadily retained on HA/PLGA‐E matrices. The wound healing activity of HA/PLGA‐E matrices is evaluated in streptozotocin‐induced diabetic rats. After two weeks of surgical treatment, the wound areas are significantly reduced by the coverage with HA/PLGA‐E matrices resulting from enhanced re‐epithelialization/neovascularization and increased collagen deposition, compared with no treatment or HA/PLGA. In conclusion, the HA/PLGA‐E matrices can be potentially exploited to craft strategies for the acceleration of diabetic wound healing and skin regeneration. Abstract : Hyaluronic acid (HA)/poly(lactic‐ co ‐glycolic acid) core/shell fiber matrices loaded with epigallocatechin‐3‐ O ‐gallate (EGCG) are developed. EGCG and HA released from the fiber matrices play synergistically effective roles in full‐thickness wound healing in diabetic rats by promoting re‐epithelialization and neovascularization. These observations have significant implications in the context of crafting strategies for the development of therapeutics for diabetic wounds.