A facile modular approach toward multifunctional supramolecular polyplexes for targeting gene delivery. Issue 43 (18th October 2016)

• Record Type: Journal Article
• Authors: Liu, Jia; Hennink, Wim E.; van Steenbergen, Mies J.; Zhuo, Renxi; Jiang, Xulin
• Is Part Of: Journal of materials chemistry. Volume 4:Issue 43(2016)
• Page Start: 7022
• Page End: 7030
• Publication Date: 2016-10-18
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c6tb01671e
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A convenient modular approach for multifunctional supramolecular self-assembly polyplexes of poly(cyclodextrin) and mono-adamantane-terminated guest polymers displaying targeting cellular uptake and transfection. Abstract : Extensive efforts and numerous methodologies have been explored to develop safe and efficient gene carriers that are capable of overcoming multiple extra- and intracellular barriers during the transfection process. However, it is a great challenge to fabricate a gene carrier system containing multiple functional components. In this work, we propose a facile modular approach to design targeted multifunctional gene delivery systems, which are based on building blocks with pre-defined functions to tackle specific barriers. These building blocks can be rationally combined and self-assemble into an integrated gene delivery system via host–guest interactions. As a proof of concept, three adamantyl-terminated targeting guest modules, adamantyl terminated RGD peptide (Ad-RGD), folate and lactobionic acid terminated PEGs (FA-PEG-Ad and LA-PEG-Ad), were synthesized, characterized, and utilized for post-functionalizing of adamantyl decorated cationic PEI based supramolecular polyplexes (PEI-Ad4 /PCD/DNA) through the free CD units of the poly(β-cyclodextran) (PCD) host module. These multifunctional targeting supramolecular polyplexes exhibited enhanced cellular uptake and excellent transfection activity in receptor-positive cells. By modulating the functional components of the supramolecular platform, we can customize the gene carriers for further research on different tissues or cells in vitro or in vivo .