Colorimetric and fluorescence probe for the detection of nano-molar lysine in aqueous medium. Issue 45 (1st November 2016)

• Record Type: Journal Article
• Authors: Adhikari, Susanta; Ghosh, Avijit; Mandal, Sandip; Guria, Subhajit; Banerjee, Prajna Paramita; Chatterjee, Ansuman; Das, Debasis
• Is Part Of: Organic & biomolecular chemistry. Volume 14:Issue 45(2016)
• Page Start: 10688
• Page End: 10694
• Publication Date: 2016-11-01
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c6ob01704e
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : A new BODIPY based lysine selective probe, THBPY, is synthesized and detects nano-molar lysine in aqueous medium, exhibiting both a visible change in color from yellow-green to orange and a change in the fluorescence profile. Abstract : A single crystal X-ray structurally characterized BODIPY based probe, THBPY, derived from 4-hydroxy-5-isopropyl-2 methyl-isophthalaldehyde, detects nano-molar lysine in aqueous medium. In the presence of lysine, THBPY visibly changes its color and fluorescence profile due to the formation of a stable imine bond. A distinctive color change allows for facile discrimination over other amino acids in a wide range of concentrations of lysine. The detection limit for lysine is 0.001 μM by a fluorescence method and 0.01 μM by a colorimetric method. The probe shows good reversibility for multiple uses and cleanly discriminates between lysine and other amino acids. Density functional theoretical studies closely resemble experimental results.