Studies of G-quadruplexes formed within self-assembled DNA mini-circles. Issue 84 (3rd October 2016)

• Record Type: Journal Article
• Authors: Klejevskaja, Beata; Pyne, Alice L. B.; Reynolds, Matthew; Shivalingam, Arun; Thorogate, Richard; Hoogenboom, Bart W.; Ying, Liming; Vilar, Ramon
• Is Part Of: Chemical communications. Volume 52:Issue 84(2016)
• Page Start: 12454
• Page End: 12457
• Publication Date: 2016-10-03
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc07110d
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We have developed self-assembled DNA mini-circles that contain a G-quadruplex-forming sequence and demonstrate by FRET that the G-quadruplex unfolding kinetics are 10-fold slower than for the simpler 24-mer G-quadruplex that is commonly used for FRET experiments. Abstract : We have developed self-assembled DNA mini-circles that contain a G-quadruplex-forming sequence from the c-Myc oncogene promoter and demonstrate by FRET that the G-quadruplex unfolding kinetics are 10-fold slower than for the simpler 24-mer G-quadruplex that is commonly used for FRET experiments.