Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography. Issue 90 (24th October 2016)

• Record Type: Journal Article
• Authors: Nagy, Gabe; Peng, Tianyuan; Kabotso, Daniel E. K.; Novotny, Milos V.; Pohl, Nicola L. B.
• Is Part Of: Chemical communications. Volume 52:Issue 90(2016)
• Page Start: 13253
• Page End: 13256
• Publication Date: 2016-10-24
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc07584c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Analytical techniques that can be coupled to automated oligosaccharide synthesis platforms are needed to purify to homogeneity protected carbohydrates at levels of ≥99.5% purity. Abstract : Given recent advances in automated oligosaccharide synthesis, analytical techniques that can be coupled to a synthetic framework are needed to not just identify but also purify to homogeneity protected carbohydrate compounds at levels of ≥99.5% purity. Herein, an alternate-pump recycling high-performance liquid chromatography (R-HPLC) method has been developed to allow purification of protected carbohydrates at levels of ≥99.5% purity.