Chloromethyl-triazole: a new motif for site-selective pseudo-acylation of proteins. Issue 82 (26th September 2016)

• Record Type: Journal Article
• Authors: Brewster, Richard C.; Gavins, Georgina C.; Günthardt, Barbara; Farr, Sarah; Webb, Kimberly M.; Voigt, Philipp; Hulme, Alison N.
• Is Part Of: Chemical communications. Volume 52:Issue 82(2016)
• Page Start: 12230
• Page End: 12232
• Publication Date: 2016-09-26
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc06801d
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Chloromethyl triazoles are shown to be cysteine selective alkylation reagents for 'near-native' post-translational modification of protein and peptide substrates. Abstract : Rapid, site-selective modification of cysteine residues with chloromethyl-triazole derivatives generates pseudo-acyl sLys motifs, mimicking important post-translational modifications. Near-native biotinylation of peptide and protein substrates is shown to be site-selective and modified histone H4 retains functional activity.