Recognition of tumor cells by immuno-SERS-markers in a microfluidic chip at continuous flow. Issue 21 (7th October 2016)

• Record Type: Journal Article
• Authors: Freitag, I.; Beleites, C.; Dochow, S.; Clement, J. H.; Krafft, C.; Popp, J.
• Is Part Of: Analyst. Volume 141:Issue 21(2016)
• Page Start: 5986
• Page End: 5989
• Publication Date: 2016-10-07
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c6an01739h
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : SERS active nanoparticles were labeled with a reporter molecule and conjugated with anti-EpCAM antibodies. Abstract : SERS active nanoparticles were labeled with a reporter molecule and conjugated with anti-EpCAM antibodies. These immuno SERS markers were mixed with leukocytes, MCF-7 breast cancer cells and polystyrene beads, and the mixture was injected into a microfluidic quartz chip. Raman spectra were acquired at 785 nm excitation with 25 milliseconds exposure time in a continuous flow regime. Spectral unmixing by N-FINDR identified spectral signatures of SERS-labelled cells and polystyrene beads. This approach demonstrated rapid and reproducible SERS-assisted cell detection. Strategies are discussed to further increase the throughput for cell sorting.