Vitamin D and/or calcium deficient diets may differentially affect muscle fiber neuromuscular junction innervation. Issue 6 (1st October 2016)

• Record Type: Journal Article
• Authors: Gifondorwa, David J.; Thompson, Tyran D.; Wiley, June; Culver, Alexander E.; Shetler, Pamela K.; Rocha, Guilherme V.; Ma, Yanfei L.; Krishnan, Venkatesh; Bryant, Henry U.
• Is Part Of: Muscle & nerve. Volume 54:Issue 6(2016)
• Page Start: 1120
• Page End: 1132
• Publication Date: 2016-10-01
• Subjects: calcium -- muscle -- neuromuscular junction -- stress response -- vitamin D; Neuromuscular diseases -- Periodicals; Muscles -- Periodicals; Nerves -- Periodicals; 616.74
• DOI: 10.1002/mus.25146
• Languages: English
• ISSNs: 0148-639X

Abstract

ABSTRACT: Introduction : There is evidence that supports a role for Vitamin D (Vit. D) in muscle. The exact mechanism by which Vit. D deficiency impairs muscle strength and function is not clear. Methods : Three‐week‐old mice were fed diets with varied combinations of Vit. D and Ca 2+ deficiency. Behavioral testing, genomic and protein analysis, and muscle histology were performed with a focus on neuromuscular junction (NMJ) ‐related genes. Results : Vit. D and Ca 2+ deficient mice performed more poorly on given behavioral tasks than animals with Vit. D deficiency alone. Genomic and protein analysis of the soleus and tibialis anterior muscles revealed changes in several Vit. D metabolic, NMJ‐related, and protein chaperoning and refolding genes. Conclusions : These data suggest that detrimental effects of a Vit. D deficient or a Vit. D and Ca 2+ deficient diet may be a result of differential alterations in the structure and function of the NMJ and a lack of a sustained stress response in muscles. Muscle Nerve 54 : 1120–1132, 2016