From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions. (October 2016)

• Record Type: Journal Article
• Authors: Nikel, Pablo I; Chavarría, Max; Danchin, Antoine; de Lorenzo, Víctor
• Is Part Of: Current opinion in chemical biology. Volume 34(2016)
• Page Start: 20
• Page End: 29
• Publication Date: 2016-10
• Subjects: Bioorganic chemistry -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Clinical biochemistry -- Periodicals; Biochemistry -- Periodicals; Chimie bio-organique -- Périodiques; Biologie -- Périodiques; 572.05
• DOI: 10.1016/j.cbpa.2016.05.011
• Languages: English
• ISSNs: 1367-5931

Abstract

Graphical abstract: Highlights: SynBio needs chassis with superior genetic, biochemical, and physiological traits. Environmental bacteria are endowed with many advantageous metabolic properties. Pseudomonas putida lifestyle is fueled by a especial metabolic architecture. The EDEMP cycle can be manipulated through contemporary metabolic engineering. Such robust background metabolism able to accommodate harsh biochemical reactions. Abstract : The soil bacterium Pseudomonas putida is endowed with a central carbon metabolic network capable of fulfilling high demands of reducing power. This situation arises from a unique metabolic architecture that encompasses the partial recycling of triose phosphates to hexose phosphates — the so-called EDEMP cycle. In this article, the value of P. putida as a bacterial chassis of choice for contemporary, industrially-oriented metabolic engineering is addressed. The biochemical properties that make this bacterium adequate for hosting biotransformations involving redox reactions as well as toxic compounds and intermediates are discussed. Finally, novel developments and open questions in the continuous quest for an optimal microbial cell factory are presented at the light of current and future needs in the area of biocatalysis.