Development of a rapid 21‐plex autosomal STR typing system for forensic applications. Issue 21 (9th May 2016)

• Record Type: Journal Article
• Authors: Yang, Meng; Yin, Caiyong; Lv, Yuexin; Yang, Yaran; Chen, Jing; Yu, Zailiang; Liu, Xu; Xu, Meibo; Chen, Feng; Wu, Huijuan; Yan, Jiangwei
• Is Part Of: Electrophoresis. Volume 37:Issue 21(2016)
• Page Start: 2789
• Page End: 2799
• Publication Date: 2016-05-09
• Subjects: Direct amplification -- DNA genotyping -- Forensic validation -- Rapid PCR -- Short tandem repeats; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201500498
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract : DNA‐STR genotyping technology has been widely used in forensic investigations. Even with such success, there is a great need to reduce the analysis time. In this study, we established a new rapid 21‐plex STR typing system, including 13 CODIS loci, Penta D, Penta E, D12S391, D2S1338, D6S1043, D19S433, D2S441 and Amelogenin loci. This system could shorten the amplification time to a minimum of 90 min and does not require DNA extraction from the samples. Validation of the typing system complied with the Scientific Working Group on DNA Analysis Methods (SWGDAM) and the Chinese National Standard (GA/T815‐2009) guidelines. The results demonstrated that this 21‐plex STR typing system was a valuable tool for rapid criminal investigation.