Nuclear incorporation of iron during the eukaryotic cell cycle. (18th October 2016)

• Record Type: Journal Article
• Authors: Robinson, Ian; Yang, Yang; Zhang, Fucai; Lynch, Christophe; Yusuf, Mohammed; Cloetens, Peter
• Is Part Of: Journal of synchrotron radiation. Volume 23:Part 6(2016)
• Page Start: 1490
• Page End: 1497
• Publication Date: 2016-10-18
• Subjects: fluorecence -- beamline -- chromosome -- imaging -- phase contrast; Synchrotron radiation -- Periodicals; Free electron lasers -- Periodicals; 539.73505
• DOI: 10.1107/S1600577516012807
• Languages: English
• ISSNs: 0909-0495

Abstract

Abstract : Scanning X‐ray fluorescence microscopy has been used to probe the distribution of S, P and Fe within cell nuclei at the new ID16 beamline. Estimates of P, S and mass signals for the chromosomal matter agree with quantitative X‐ray phase contrast projection microscopy images of the same samples, while fluorescence shows Fe incorporation. Abstract : Scanning X‐ray fluorescence microscopy has been used to probe the distribution of S, P and Fe within cell nuclei. Nuclei, which may have originated at different phases of the cell cycle, are found to show very different levels of Fe present with a strongly inhomogeneous distribution. P and S signals, presumably from DNA and associated nucleosomes, are high and relatively uniform across all the nuclei; these agree with X‐ray phase contrast projection microscopy images of the same samples. Possible reasons for the Fe incorporation are discussed.