Detection of interleukin-2 is not useful for distinguishing between latent and active tuberculosis in clinical practice: a prospective cohort study. (December 2016)

• Record Type: Journal Article
• Authors: Struzka, Edu A.; Climent, Joan; Penas-Truque, Antón; Pallarés-Sanmartín, Abel; Ríos, Mónica; Pargada-Ferrer, Diego J.; Agüero-Balbin, Jesús; Rodríguez-Gutiérrez, Juan F.; Santin, M.; Morandeira-Rego, F.; Alcaide, F.; Rabuñal, R.; Anibarro, L.; Agüero-Balbín, R.; Casas-Garcia, X.; Pérez-Escolano, E.; Navarro, M.D.; Sánchez, F.; Coira-Nieto, A.; Trigo-Daporta, M.; Martinez-Meñaca, A.; Gonzalez-Cuevas, A.; López-Prieto, M.D.; Domínguez-Castellano, A.; Jové, N.
• Is Part Of: Clinical microbiology and infection. Volume 22:Number 12(2016)
• Page Start: 1007.e1
• Page End: 1007.e5
• Publication Date: 2016-12
• Subjects: Active tuberculosis -- Interferon-γ -- Interleukin-2 -- Latent tuberculosis infection -- QuantiFERON; Medical microbiology -- Periodicals; Diagnostic microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.01
• DOI: 10.1016/j.cmi.2016.09.004
• Languages: English
• ISSNs: 1198-743X

Abstract

Abstract: Objective: Previous reports have identified interleukin-2 (IL-2), quantified in the supernatants of QuantiFERON ® -TB Gold In-tube (QFT) after 72 h of incubation, as a potential biomarker for distinguishing between latent and active tuberculosis (TB). However, its validity has not been tested in an appropriate clinical cohort. Methods: A multicentre study of 161 consecutive adult patients undergoing evaluation for active TB at eight TB Units in Spain. Interferon-γ (IFN-γ) and IL-2 were assessed in the supernatant of QFT after 16–24 h and 72 h of incubation. The accuracy of IL-2 for indicating latent TB infection (LTBI) was assessed by receiving operating characteristic curves. . Results: Twenty-eight participants were not infected, 43 had LTBI, 69 had TB, and 21 were not classifiable. Median (interquartile range) IL-2 concentrations after 72 h of incubation were 0.0 pg/mL (0.0–0.0) in uninfected individuals, 261.0 pg/mL (81.0–853.0) in LTBI individuals, 166.5 pg/mL (33.5–551.5) in patients with extrapulmonary TB, 95.0 pg/mL (26.0–283.0) in patients with smear-negative pulmonary TB, and 38.5 pg/mL (7.5–178.0) in patients with smear-positive pulmonary TB (p <0.0001). The area under the curve of the receiving operating characteristic curve (95% CI) of IL-2 after 72 h of incubation for the diagnosis of LTBI was 0.63 (0.53–0.74) when all TB cases were considered as a single group, ranging from 0.59 (0.47–0.71) to 0.72 (0.58–0.85) when only extrapulmonary and smear-positive pulmonary TB cases respectively were considered. Conclusions: Quantification of IL-2 in the supernatant of QFT after a prolonged incubation is not useful to distinguish between LTBI and active disease in clinical practice.