7, 8-Dihydroxyflavone reverses the depressive symptoms in mouse chronic mild stress. (2nd December 2016)

• Record Type: Journal Article
• Authors: Zhang, Min-Wang; Zhang, She-feng; Li, Zhen-Hua; Han, Fang
• Is Part Of: Neuroscience letters. Volume 635(2016)
• Page Start: 33
• Page End: 38
• Publication Date: 2016-12-02
• Subjects: 7, 8-Dihydroxyflavone (7, 8-DHF) -- Antidepressant-like effects -- Chronic mild stress (CMS) -- TrkB -- PSD95 -- Synaptophysin; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2016.10.035
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: 7, 8-DHF produced antidepressant-like effects in CMS mice. 7, 8-DHF promoted the expression of synaptic proteins. K252a pretreatment blocked the effects of 7, 8-DHF. Abstract: 7, 8-Dihydroxyflavone (7, 8-DHF) is a naturally-occurring flavone which possesses good bioavailability. Due to its ability to cross the blood-brain barrier, previous studies have demonstrated that 7, 8-DHF was a potent tropomyosin-related kinase B (TrkB) agonist, and produced antidepressant-like effects in mouse forced swimming test and tail suspension test. However, it has not been evaluated in chronic mild stress (CMS), a classical depression model modulating the processes of major depression in human. In the present study, we not only evaluated the depressive-like behaviors, but also measured the key proteins of TrkB signaling in mice exposed to CMS. Our results firstly found that long term but not single injection of 7, 8-DHF restored the depressive-like behaviors in sucrose preference test and novelty suppressed feeding test. In addition, 7, 8-DHF not only increased TrkB phosphorylation and brain-derived neurotrophic factor (BDNF) levels, but also activated the expression of TrkB downstream synaptic proteins such as PSD95 and synaptophysin. Furthermore, the TrkB antagonist K252a blocked the antidepressant-like effects of 7, 8-DHF. In summary, the present results demonstrated that chronic 7, 8-DHF treatment exerted significant antidepressant-like effects, which were likely attributed to regulating TrkB signaling and thus promoting synaptic protein expression.