Crabapple fruit extracts lower hypercholesterolaemia in high-fat diet-induced obese mice. (December 2016)

• Record Type: Journal Article
• Authors: Li, Mingxia; Xue, Shuangjia; Tan, Si; Qin, Xiaoxiao; Gu, Ming; Wang, Dongshan; Zhang, Yu; Guo, Lu; Huang, Feisi; Yao, Yunchong; Zhou, Zhiqin; Fan, Shengjie; Huang, Cheng
• Is Part Of: Journal of functional foods. Volume 27(2016)
• Page Start: 416
• Page End: 428
• Publication Date: 2016-12
• Subjects: Crabapple -- Hyperlipidaemia -- Hypercholesterolaemia -- Metabolic syndrome -- Anti-oxidation -- CYP7A1; Procyanidin (PubChem CID: 107876) -- Epicatechin (PubChem CID: 72276) -- Isoastilbin (PubChem CID: 10114809) -- Quercetin 3-O-glucoside (PubChem CID: 44258003) -- Avicularin (PubChem CID: 5490064) -- Quercetin 3-O-rhamnoside (PubChem CID: 5280459) -- Phlorizin (PubChem CID: 6072) -- Phloretin (PubChem CID: 4788) -- Quercetin (PubChem CID: 5280343) -- 3-Caffeoylquinic acid (PubChem CID: 1794427); ABCA1 ATP-binding cassette transporter A1 -- ABTS 2, 2′-azino-bis(3-ethylbenzthiozoline-6)-sulphonic acid -- ACO acyl-CoA oxidase -- ACC acetyl-CoA carboxylase -- aP2 adipocyte fatty acid-binding protein -- AUC area under the curve -- COX cyclooxygenase -- CYP7A1 cholesterol 7 alpha-hydroxylase -- DIO diet-induced obesity -- DO M. cv. Donald -- DPPH 2, 2-diphenyl-1-picrylhydrazyl -- FRAP ferric reducing antioxidant power -- HDL-c high-density lipoprotein cholesterol -- HMG-CoA 3-hydroxy-3-methyl-glutaryl-coenzyme A -- IM Indian Magic -- LDL-c low-density lipoprotein cholesterol -- LDLR low density lipoprotein receptor -- LPL lipoprotein lipase -- LXR liver X receptor -- PB M. prunifolia Borkh -- PPAR peroxisome proliferator-activated receptor -- RS M. cv. Red Splendor -- TC total cholesterol -- TG triglyceride -- XJ M. xaojinensis Cheng et Jiang; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.09.017
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Crabapple extracts lowered TC and LDL-c levels. Crabapple extracts elevated the mRNA and protein levels of CYP7A1 in mouse liver. Crabapple extracts displayed antioxidant activity. Abstract: Crabapples are used as food and for the treatment of diarrhoea, indigestion and other digestive diseases in China. The anti-hyperlipidaemic and anti-hyperglycaemic effects of the extracts were evaluated on high-fat diet-induced obese mice. We found that the extracts of five crabapple varieties lowered serum total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-c) levels, and decreased serum triglyceride (TG) contents and improved fasting glucose levels in the mice. All extracts notably elevated the mRNA expression and protein levels of cholesterol 7 alpha-hydroxylase (CYP7A1), the crucial enzyme for cholesterol homeostasis, in the mouse liver. The extracts also displayed strong antioxidant activity in vitro . To our knowledge, this is the first study to show that the crabapples could lower cholesterol levels. Our data suggest that crabapple may lower cholesterol through the enhancement of CYP7A1 function in liver, and crabapple may be used as nutraceuticals to prevent or treat hyperlipidaemia and other metabolic disorders.