In vitro studies of an aged black garlic extract enriched in S-allylcysteine and polyphenols with cardioprotective effects. (December 2016)

• Record Type: Journal Article
• Authors: García-Villalón, A.L.; Amor, S.; Monge, L.; Fernández, N.; Prodanov, M.; Muñoz, M.; Inarejos-García, A.M.; Granado, M.
• Is Part Of: Journal of functional foods. Volume 27(2016)
• Page Start: 189
• Page End: 200
• Publication Date: 2016-12
• Subjects: Aged black garlic -- Ischaemia–reperfusion -- Nitric oxide -- Antioxidant -- Anti-inflammatory; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.08.062
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Aged black garlic prevents ischaemia–reperfusion induced alterations in the heart. Aged black garlic induces vasodilation specifically in coronary arteries. Aged black garlic induces the overexpression of pro- and anti-inflammatory markers. Aged black garlic induces the overexpression of pro- and anti-oxidant markers. Aged black garlic improves cardiovascular function through hormesis mechanism. Abstract: Aged black garlic (ABG) exerts metabolic and cardiovascular beneficial effects. The aim of this work was to analyse the in vitro cardiovascular effects of an ABG extract enriched in S -allyl-cysteine and polyphenols (ABG10+) in Sprague-Dawley rats. Hearts were pre-treated either with ABG10+ or vehicle and subjected to 30 min ischaemia followed by 45 min reperfusion (IR) using the Langendorff technique. Segments of aorta and tail artery were used for inflammation/oxidative stress and vascular reactivity experiments respectively in presence/absence of ABG10+. ABG10+ induced a nitric oxide (NO) dependent vasodilating effect in tail artery segments and directly increased the release of NO in aorta segments. In the heart, ABG10+ induced a relaxing effect on coronary arteries before and after IR and prevented the IR induced decrease in myocardial contractility. Functional changes were associated with increased expression of both pro- and antioxidant and pro- and anti-inflammatory markers in the myocardium and in aorta.