Potential chemopreventive activities of a polyphenol rich purified extract from olive mill wastewater on colon cancer cells. (December 2016)

• Record Type: Journal Article
• Authors: Bassani, Barbara; Rossi, Teresa; De Stefano, Daniela; Pizzichini, Daniele; Corradino, Paola; Macrì, Nicoletta; Noonan, Douglas M.; Albini, Adriana; Bruno, Antonino
• Is Part Of: Journal of functional foods. Volume 27(2016)
• Page Start: 236
• Page End: 248
• Publication Date: 2016-12
• Subjects: Olive mill wastewater -- Polyphenols -- Hydroxytyrosol -- Chemoprevention -- Colon cancer; 7-AAD 7-amino-actinomycin D -- COD chemical oxygen demand -- CC colon cancer -- DAD diode array detector -- ECM extracellular matrix -- FACS fluorescence-activated cell sorting -- FSC forward scatter -- FITC fluorescein isothiocyanate -- 5-FU 5-fluorouracil -- FBS foetal bovine serum -- HPLC high-performance liquid chromatography -- HyT hydroxytyrosol -- LC liquid chromatography -- MS mass spectrometry -- MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide -- OMWW olive mill wastewater -- PFA paraformaldehyde -- SSC side scatter -- UV–vis ultraviolet–visible -- VCR volume concentration ratio; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.09.009
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Olive mill wastewater (OMWW) is a waste product of olive oil, rich in polyphenols. A009 is a water soluble polyphenol extract from OMWW. Actions of polyphenols may be beneficial in preventing colon cancer. A009 inhibits growth, migration, invasion and adhesion of colon cancer cells. A009 inhibits tumour growth in vivo with promising cancer preventive properties. Abstract: Olive oil, a major feature of the Mediterranean diet, is an abundant source of phenolic compounds. Olive oil production is associated with the generation of waste material, termed 'olive mill wastewater' (OMWW), that has been reported to be enriched in soluble polyphenols. Given the known beneficial activity of polyphenols, we investigated whether the use of purified extracts from OMWW, termed A009, rich in hydroxytyrosol, might have anticancer activities on colon cancer (CC) cell lines in vitro and in vivo and could represent a chemopreventive preparation for CC. A009 from different batches inhibited proliferation, migration, invasion, adhesion, sprouting of CC cells and release of angiogenic, pro-inflammatory cytokines (VEGF, IL-8). Our data demonstrate that a novel purified, polyphenol enriched extract, obtained from food industry waste material, with similar activity than purified hydroxytyrosol but easier to produce in large quantities and with an environment-sensitive approach, has potential cancer chemopreventive properties for colon cancer cells.