Common bean (Phaseolus vulgaris L.) protein-derived peptides increased insulin secretion, inhibited lipid accumulation, increased glucose uptake and reduced the phosphatase and tensin homologue activation in vitro. (December 2016)

• Record Type: Journal Article
• Authors: Oseguera Toledo, Miguel E.; Gonzalez de Mejia, Elvira; Sivaguru, Mayandi; Amaya-Llano, Silvia L.
• Is Part Of: Journal of functional foods. Volume 27(2016)
• Page Start: 160
• Page End: 177
• Publication Date: 2016-12
• Subjects: Diabetes -- Hard to cook bean (Phaseolus vulgaris) -- Hydrolysates -- Bioactive peptides -- Insulin -- Glucose uptake; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.09.001
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Peptides from pinto and black beans increased glucose-stimulated insulin secretion. Common bean peptides have a cytoprotective effect against intracellular ROS. Bean peptides inhibited adipocyte lipid accumulation and increased glucose uptake. Bean peptides reduced DPP-IV and RAGE expression and oxygen species. Abstract: The objective of this study was to evaluate and compare the effect of peptides produced from hard-to-cook common bean proteins on insulin secretion from pancreatic β-cells and glucose uptake from insulin-resistant adipocytes, and to understand the mechanism of action on markers related to glucose metabolism, in vitro . Hydrolysates and peptide fractions < 1 kDa of two common bean varieties (pinto Durango and black 8025) were produced with alcalase and bromelain, and were further hydrolysed with pepsin-pancreatin to simulate gastrointestinal tract digestion. All bean treatments were able to increase glucose-stimulated insulin secretion from rat insulinoma INS-1E cells, reduced the expression of proteins like dipeptidyl peptidase IV (DPP-IV) and receptor for advanced glycation end products (RAGE) and significantly reduced oxygen species (up to 70%). Peptides also inhibited lipid accumulation in mature adipocytes 3T3-L1 and increased glucose uptake (67%) via Akt modulation in insulin resistant adipocytes enhancing insulin signalling and reducing the phosphatase and tensin homologue (PTEN) activation.