Protective effect of kaempferol, a flavonoid widely present in varieties of edible plants, on IL-1β-induced inflammatory response via inhibiting MAPK, Akt, and NF-κB signalling in SW982 cells. (December 2016)

• Record Type: Journal Article
• Authors: Lian, Jun-Jiang; Cheng, Bin-Feng; Gao, Yao-Xin; Xue, Han; Wang, Lei; Wang, Mian; Yang, Hai-Jie; Feng, Zhi-Wei
• Is Part Of: Journal of functional foods. Volume 27(2016)
• Page Start: 214
• Page End: 222
• Publication Date: 2016-12
• Subjects: Kaempferol -- Inflammatory mediator -- Signalling pathway -- SW982 cells; COX cyclooxygenase -- MMP matrix metallo proteinase -- RA rheumatoid arthritis -- TNF-α tumour necrosis factor-α -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- AMPK adenosine monophosphate-activated protein kinase -- MAPK mitogen-activated protein kinase -- PI3K phosphatidylinositol 3-kinase -- Akt protein kinase B -- ERK extracellular signal-related kinase -- JNK c-Jun N-terminal kinase -- NF-κB nuclear factor-κB; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.09.003
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Anti-inflammatory effects and mechanisms of kaempferol were evaluated in SW982 cells. First report about the effects of kaempferol on IL-6 and IL-8 in human synovial cells. The phosphorylations of ERK, p38, JNK and Akt were inhibited. IκB-α degradation and p65 translocation into nucleus were decreased. AMPK signalling does not contribute to the anti-inflammatory effects of kaempferol. Abstract: Effects and mechanisms of kaempferol, identified in a wide variety of dietary sources, on the expression of interleukin (IL)-6, IL-8, matrix metallo proteinase (MMP)-1, and cyclooxygenase (COX)-2 were evaluated in IL-1β-induced SW982 cells. The cells were pretreated with kaempferol of various concentrations (2, 10 and 50 µmol/L) followed by IL-1β (10 ng/mL) stimulation. Kaempferol significantly suppressed mRNA and protein levels of IL-6, IL-8, MMP-1, and COX-2 induced by IL-1β. Kaempferol also inhibited mitogen-activated protein kinase (MAPK) and protein kinase B (Akt) phosphorylation and decreased IκB-α degradation and p65 protein translocation into the nucleus. Moreover, adenosine monophosphate-activated protein kinase (AMPK) does not contribute to the anti-inflammatory effects in SW982 cells, although its phosphorylation is significantly increased by kaempferol. These results indicate that kaempferol may have an anti-inflammatory effect by inhibiting expression of IL-6, IL-8, MMP-1 and COX-2 via suppressing MAPK, Akt, and nuclear factor (NF)-κB signalling but not activating AMPK signalling.