Catabolism of raw and cooked green pepper (Capsicum annuum) (poly)phenolic compounds after simulated gastrointestinal digestion and faecal fermentation. (December 2016)

• Record Type: Journal Article
• Authors: Juániz, Isabel; Ludwig, Iziar Amaia; Bresciani, Letizia; Dall'Asta, Margherita; Mena, Pedro; Del Rio, Daniele; Cid, Concepcion; de Peña, María-Paz
• Is Part Of: Journal of functional foods. Volume 27(2016)
• Page Start: 201
• Page End: 213
• Publication Date: 2016-12
• Subjects: Polyphenols -- In vitro bioaccessibility -- In vitro gastrointestinal digestion -- Colonic catabolism -- Heat treatment -- Pepper; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.09.006
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Heat treatment exerts a positive effect on the bioaccessibility of pepper (poly)phenols. (Poly)phenols remain 96–100% bioaccessible in olive and sunflower oil fried peppers. Griddled pepper showed the highest bioaccessibility of (poly)phenols after digestion. Catabolic pathways for degradation of pepper (poly)phenols in the colon are proposed. Griddled pepper showed the highest amount of (poly)phenols after faecal fermentation. Abstract: A total of 21 (poly)phenolic compounds (free and bound) were quantified in raw, olive oil fried, sunflower oil fried and griddled green pepper before and after a simulated gastrointestinal digestion. Flavonoids, particularly quercetin rhamnoside, were the main compounds. The bioaccessibility of (poly)phenolic compounds after gastrointestinal digestion was higher in cooked (>82%) than in raw (48%) samples, showing a positive effect of heat treatment on the release of (poly)phenols from the vegetal matrix. Additionally, a faecal fermentation was carried out for 24h. A time-dependent microbial metabolic activity was observed, which resulted firstly (<5h) in the hydrolysis of flavonoid glycosides and then in the formation of 3 catabolites, namely 3, 4-dihydroxybenzoic acid, dihydrocaffeic acid and 3-(3′-hydroxyphenyl)propionic acid, this being by far the most abundant. Catabolic pathways for colonic microbial degradation of flavonoids and hydroxycinnamic acids have been proposed. Griddled pepper showed the highest amount of (poly)phenols both after gastrointestinal digestion and colonic fermentation.