Beneficial effect of Ageratum conyzoides Linn (Asteraceae) upon inflammatory response induced by carrageenan into the mice pleural cavity. (24th December 2016)

• Record Type: Journal Article
• Authors: Vigil de Mello, Silvana Virgínia Gagliotti; da Rosa, Julia Salvan; Facchin, Bruno Matheus; Luz, Ana Beatriz Gobbo; Vicente, Geison; Faqueti, Larissa Gabriela; Rosa, Daniela Weingartner; Biavatti, Maique Weber; Fröde, Tânia Silvia
• Is Part Of: Journal of ethnopharmacology. Volume 194(2016)
• Page Start: 337
• Page End: 347
• Publication Date: 2016-12-24
• Subjects: ADA adenosine deaminase -- A. conyzoides L. Ageratum conyzoides Linn. -- CBA cytometric bead array -- CONCEA National Council of Animal Experimentation Control -- CE crude extract -- DCM-F dichloromethane sub-fraction -- Dex dexamethasone -- EIA enzyme immunoassay -- ESI electrospray ionization -- EtOH-F ethanol fraction -- EtOAc-F ethyl acetate sub-fraction -- HEX-F hexane fraction -- IFN-γ interferon gamma -- IL interleukin -- iNOS inducible nitric oxide synthase -- i.p. intraperitoneal -- i.pl. intrapleural -- i.v. intravenous -- MAPK mitogen-activated protein kinases -- MeONOB 5′-methoxy nobiletin -- MPO myeloperoxidase -- NF-κB factor nuclear kappa B -- NMR nuclear magnetic resonance -- NO nitric oxide -- NOx nitric oxide metabolites -- No. SMDB Herbarium Santa Maria Department of Biology -- PBS phosphate buffered saline -- p-p38 MAPK p38 mitogen-activated protein kinases -- p-p65 NF-κB phosphorylation of p65 subunit of NF-κB -- ROS reactive oxygen species -- Th T helper -- TNF tumor necrosis factor -- UPLC-HRMS ultra performance liquid chromatography coupled to high-resolution mass spectrometry; 5′-Methoxy nobiletin (PubChem CID: 72815) -- 1, 2-Benzopyrone (PubChem CID: 323) -- Eupalestin (PubChem CID: 331149); Ageratum conyzoides Linn (Asteraceae) -- Anti-inflammatory effects -- Pro-inflammatory mediators -- NF-Kappa B -- MAPK; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2016.09.003
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Ageratum conyzoides Linn (Asteraceae), a tropical plant that is very common in West Africa and some parts of Asia and South America, has been used to treat inflammatory disorders. In Brazil, teas made from A. conyzoides L. are used as anti-inflammatory, analgesic and anti-diarrheic agents. Therefore, it is necessary to study the mechanism of anti-inflammatory action of A. conyzoides L. to support its medicinal use for treating inflammatory conditions. These studies will also support the development of effective pharmacological agents with potent anti-inflammatory properties. Aim of the study: To evaluate the anti-inflammatory effects of the crude extract (CE), its derived fractions: ethanol (EtOH-F), hexane (HEX-F), ethyl acetate (EtOAc-F) and dichloromethane (DCM-F) and isolated compounds, such as 5′-methoxy nobiletin (MeONOB), 1, 2-benzopyrone and eupalestin, which are obtained from the aerial parts of A. conyzoides L. Materials and methods: These evaluations were performed using an animal model of inflammation induced by carrageenan. The following inflammatory parameters were analysed: leukocyte influx, protein concentration of the exudate, myeloperoxidase (MPO), adenosine deaminase (ADA) and nitric oxide metabolites (NOx) concentrations, interleukin 10 (IL-10), interleukin 17A (IL-17A), interleukin 6 (IL-6), tumor necrosis factor (TNF), interferon gamma (IFN-γ) and phosphorylation of p65 subunit of NF-κB (p-p65 NF-κB), p38 mitogen-activated protein kinases (p-p38 MAPK) were also analysed. Results: CE, its EtOH-F, HEX-F, EtOAc-F and DCM-F and the isolated compounds, including MeONOB, 1, 2-benzopyrone and eupalestin, significantly reduced leukocyte influx, protein concentration of the exudate, MPO, ADA, and NOx concentrations ( p <0.05). CE, EtOH-F and isolated compounds significantly reduced IL-17A, IL-6, TNF and IFN-γ levels ( p <0.05). CE, EtOH-F and isolated compound 1, 2-benzopyrone also increased IL-10 levels ( p <0.05). Isolated compounds, MeONOB, 1, 2-benzopyrone and eupalestin, reduced p-p65 NF-κB and p-p38 MAPK ( p <0.01). Conclusions: This study demonstrates that A. conyzoides L. exerts its important anti-inflammatory properties by inhibiting leukocyte influx and protein concentration of the exudate, as well as reducing the levels of several pro-inflammatory mediators. The anti-inflammatory action of A. conyzoides L. may be because of the inhibition of p65 NF-κB and MAPK activation by the isolated compounds. Graphical abstract: