Structural and functional damages of whole body ionizing radiation on rat brain homogenate membranes and protective effect of amifostine. (1st December 2016)

• Record Type: Journal Article
• Authors: Cakmak, Gulgun; Severcan, Mete; Zorlu, Faruk; Severcan, Feride
• Is Part Of: International journal of radiation biology. Volume 92:Number 12(2016:Dec.)
• Page Start: 837
• Page End: 848
• Publication Date: 2016-12-01
• Subjects: Amifostine -- brain homogenate membrane -- FTIR spectroscopy -- ionizing radiation -- lipid peroxidation -- protein secondary structure; Radiation -- Physiological effect -- Periodicals; Radiobiology -- Periodicals; 571.45
• DOI: 10.1080/09553002.2016.1230237
• Languages: English
• ISSNs: 0955-3002

Abstract

Abstract: Purpose: To investigate the effects of whole body ionizing radiation at a sublethal dose on rat brain homogenate membranes and the protective effects of amifostine on these systems at molecular level. Materials and methods: Sprague-Dawley rats, in the absence and presence of amifostine, were whole-body irradiated at a single dose of 8 Gy and decapitated after 24 h. The brain homogenate membranes of these rats were analyzed using Fourier Transform Infrared (FTIR) spectroscopy. Results: Ionizing radiation caused a significant increase in the lipid to protein ratio and significant decreases in the ratios of olefinic = CH/lipid, CH2 /lipid, carbonyl ester/lipid and CH3 /lipid suggesting, respectively, a more excessive decrease in the protein content and the degradation of lipids as a result of lipid peroxidation. In addition, radiation changed the secondary structure of proteins and the status of packing of membrane lipid head groups. Furthermore, it caused a decrease in lipid order and an increase in membrane fluidity. The administration of amifostine before ionizing radiation inhibited all the radiation-induced alterations in brain homogenate membranes. Conclusions: The results revealed that whole body ionizing radiation at a sublethal dose causes significant alterations in the structure, composition and dynamics of brain homogenate membranes and amifostine has a protective effect on these membranes.