Long term betaine supplementation regulates genes involved in lipid and cholesterol metabolism of two muscles from an obese pig breed. (February 2017)

• Record Type: Journal Article
• Authors: Albuquerque, A.; Neves, José A.; Redondeiro, M.; Laranjo, M.; Félix, M.R.; Freitas, Amadeu; Tirapicos, José L.; Martins, José M.
• Is Part Of: Meat science. Volume 124(2017)
• Page Start: 25
• Page End: 33
• Publication Date: 2017-02
• Subjects: ACC Acetyl-CoA carboxylase -- ADPN adiponectin -- AL Alentejano -- BW body weight -- C commercial -- CB C diet supplemented with betaine -- FA fatty acid -- FASN Fatty acid synthase -- H-FABP Heart-type fatty acid binding protein -- HMGCR 3-Hydroxy-3-methyl-glutaryl-CoA reductase -- HPRT1 hypoxanthine phosphoribosyltransferase 1 -- HSL Hormone-sensitive lipase -- IMF intramuscular fat -- LDLr Low-Density Lipoprotein Receptor -- LPL Lipoprotein lipase -- MCPT1 Muscle-type carnitine palmitoyltransferase 1 -- MUFA monounsaturated fatty acid -- PPARα Peroxisome proliferator-activated receptor alpha -- PPARγ Peroxisome proliferator-activated receptor gamma -- PUFA polyunsaturated fatty acid -- SCD Stearoyl-CoA desaturase -- SFA saturated fatty acid -- TAG triacylglycerol; Betaine -- Longissimus lumborum -- Biceps femoris -- Intramuscular fat and cholesterol content -- Gene expression -- Alentejano pig; Meat -- Periodicals; Meat industry and trade -- Periodicals; Viande -- Périodiques; Viande -- Industrie -- Périodiques; Meat; Meat industry and trade; Periodicals; 641.36
• DOI: 10.1016/j.meatsci.2016.10.012
• Languages: English
• ISSNs: 0309-1740

Abstract

Abstract: This study evaluates the effects of betaine supplementation (1 g kg − 1 for 20 weeks) on the regulation of genes involved in lipid and cholesterol metabolism of Longissimus lumborum and Biceps femoris from obese Alentejano pigs. Betaine supplementation led to an increase in total cholesterol in both muscles, complementing results previously published indicating a significant increase on the intramuscular lipid content. The expression of twelve genes involved in lipogenesis, lipolysis/FA oxidation, FA transport, and cholesterol metabolism, as well as two transcription factors were also evaluated. Genes related to lipid and cholesterol synthesis plus FA transport were consistently up-regulated in both muscles of betaine fed pigs. On the other hand, genes related to lipolysis/FA oxidation were not affected or down-regulated by betaine supplementation. Our data suggest that the underlying mechanism regulating IMF and cholesterol accumulation in Alentejano pigs supplemented with betaine is associated with the up-regulation of genes involved in lipid synthesis, FA transport, and cholesterol synthesis.