Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection. (November 2016)

• Record Type: Journal Article
• Authors: Costa, Juan Gabriel; Duré, Andrea Belén
• Is Part Of: Microbial pathogenesis. Volume 100(2016)
• Page Start: 229
• Page End: 236
• Publication Date: 2016-11
• Subjects: GRA8 -- Toxoplasmosis -- Acute infection -- Serodiagnosis -- Antigen -- Bioinformatics; T. gondii Toxoplasma gondii -- IgG immunoglobulin G -- IgA immunoglobulin A -- IgM immunoglobulin M -- Ag antigen -- IFI indirect immunofluorescence -- NIS negative infection sera -- CIS chronic infection sera -- AvI avidity index -- AIS acute infection sera -- PIS positive infection sera -- E. coli Escherichia coli -- PAGE polyacrylamide gel electrophoresis -- ROC Receiver Operating Characteristic -- SD standard deviations -- CfI Confidence interval; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2016.09.021
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: In this work, two Toxoplasma gondii GRA8 protein sequences were tested by indirect ELISA and measurement of avidity to differentiate between acute and chronic toxoplasmosis infection. Using the antigen called GRA8B, 79.7% sensitivity and 84.1% specificity was achieved detecting IgG concentrations and a 71.2% sensitivity and a 68.3% specificity detecting IgA concentrations. This study is the first to report IgA detection with GRA8 by ELISA to differentiate stages of infection. Unfortunately the indirect ELISA to detect IgM was not effective in distinguishing stages. Also, this work is the first to report that the GRA8 protein can aid the differentiation between acute and chronic phase infection by measuring IgG antibody avidity, a technique in which we obtained 85.71% and 100% of sensitivity and specificity, respectively. Finally, in silico tools were used to explain the differences in our immunochemistry results. Highlights: T. gondii GRA8 sequences were tested to differentiate between infection phases. Two GRA8 antigen sequences were evaluated by ELISA to detect IgG, IgA and IgM. GRA8B antigen was tested by measuring IgG avidity.