Assessing the reliability of thermography to infer internal body temperatures of lizards. (December 2016)

• Record Type: Journal Article
• Authors: Barroso, Frederico M.; Carretero, Miguel A.; Silva, Francisco; Sannolo, Marco
• Is Part Of: Journal of thermal biology. Volume 62(2016)Part A
• Page Start: 90
• Page End: 96
• Publication Date: 2016-12
• Subjects: Thermography -- Thermal imaging -- Infrared camera -- Lizard body temperature -- Regional heterothermy; Thermobiology -- Periodicals; Temperature -- Periodicals; Biology -- Periodicals; Thermobiologie -- Périodiques; Thermobiology; Periodicals; 571.46
• DOI: 10.1016/j.jtherbio.2016.10.004
• Languages: English
• ISSNs: 0306-4565

Abstract

Abstract: For many years lizard thermal ecology studies have relied on the use of contact thermometry to obtain internal body temperature (Tb ) of the animals. However, with progressing technology, an interest grew in using new, less invasive methods, such as InfraRed (IR) pyrometry and thermography, to infer Tb of reptiles. Nonetheless few studies have tested the reliability of these new tools. The present study tested the use of IR cameras as a non-invasive tool to infer Tb of lizards, using three differently body-sized lacertid species ( Podarcis virescens, Lacerta schreiberi and Timon lepidus ). Given the occurrence of regional heterothermy, we pairwise compared thermography readings of six body parts (snout, eye, head, dorsal, hind limb, tail base) to cloacal temperature (measured by a thermometer-associated thermocouple probe) commonly employed to measure Tb in field and lab studies. The results showed moderate to strong correlations (R 2 =0.84–0.99) between all body parts and cloacal temperature. However, despite the readings on the tail base showed the strongest correlation in all three species, it was the eye where the absolute values and pattern of temperature change most consistently followed the cloacal measurements. Hence, we concluded that the eye would be the body location whose IR camera readings more closely approximate that of the animal's internal environment. Alternatively, other body parts can be used, provided that a careful calibration is carried out. We provide guidelines for future research using thermography to infer Tb of lizards. Highlights: Thermography gives insight to superficial, but not internal temperature of animals. Here we compared IR with standard cloacal measurements to assess their relationship. Different body-sized species respond differently in terms of thermal heterogeneity. Different body parts correlate differently to cloacal temperature. We suggest to use eye as proxy for internal temperature of lizards whenever feasible.