Cytotoxic effects of composite dust on human bronchial epithelial cells. Issue 12 (December 2016)

• Record Type: Journal Article
• Authors: Cokic, Stevan M.; Hoet, Peter; Godderis, Lode; Wiemann, Martin; Asbach, Christof; Reichl, Franz X.; De Munck, Jan; Van Meerbeek, Bart; Van Landuyt, Kirsten L.
• Is Part Of: Dental materials. Volume 32:Issue 12(2016)
• Page Start: 1482
• Page End: 1491
• Publication Date: 2016-12
• Subjects: Dental composite -- Cytotoxicity -- Nano-particle -- Composite dust -- Human bronchial cells -- TEM; Dentistry -- Periodicals; Dental materials -- Periodicals; 617.695
• DOI: 10.1016/j.dental.2016.09.010
• Languages: English
• ISSNs: 0109-5641

Abstract

Abstract: Introduction: Previous research revealed that during routine abrasive procedures like polishing, shaping or removing of composites, high amounts of respirable dust particles (<5 μm) including nano-sized particles (<100 nm) may be released. Objective: To determine the cytotoxic potential of composite dust particles on bronchial epithelium cells. Methods: Composite dust of five commercial composites (one nano-composite, two nano-hybrid and two hybrid composites) was generated following a clinically relevant protocol. Polymerized composite samples were cut with a rough diamond bur (grain size 100 μm, speed 200, 000 rpm) and all composite dust was collected in a sterile chamber. Human bronchial epithelial cells (16HBE14o-) were exposed to serially diluted suspensions of composite dust in cell culture medium at concentrations between 1.1 and 3.3 mg/ml. After 24 h-exposure, cell viability and membrane integrity were assessed by the WST-1 and the LDH leakage assay, respectively. The release of IL-1β and IL-6 was evaluated. The composite dust particles were characterized by transmission electron microscopy and by dynamic and electrophoretic light scattering. Results: Neither membrane damage nor release of IL-1β was detected over the complete concentration range. However, metabolic activity gradually declined for concentrations higher than 660 μg/ml and the release of IL-6 was reduced when cells were exposed to the highest concentrations of dust. Significance: Composite dust prepared by conventional dental abrasion methods only affected human bronchial epithelial cells in very high concentrations.