First next-generation sequencing full-genome characterization of a hepatitis C virus genotype 7 divergent subtype. (November 2016)

• Record Type: Journal Article
• Authors: Salmona, M.; Caporossi, A.; Simmonds, P.; Thélu, M.-A.; Fusillier, K.; Mercier-Delarue, S.; De Castro, N.; LeGoff, J.; Chaix, M.-L.; François, O.; Simon, F.; Morand, P.; Larrat, S.; Maylin, S.
• Is Part Of: Clinical microbiology and infection. Volume 22:Number 11(2016)
• Page Start: 947.e1
• Page End: 947.e8
• Publication Date: 2016-11
• Subjects: Democratic Republic of Congo -- Genotype 7b -- Hepatitis C virus -- Next-generation sequencing -- Polymorphism; Medical microbiology -- Periodicals; Diagnostic microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.01
• DOI: 10.1016/j.cmi.2016.07.032
• Languages: English
• ISSNs: 1198-743X

Abstract

Abstract: We report the near-full-length genome sequence of a hepatitis C virus (HCV) isolate from a man originating from Democratic Republic of Congo, the genotype of which could not be determined by the routinely used sequencing technique. The near-complete genome sequence of this variant BAK1 was obtained by the association of two next-generation sequencing technologies. Evolutionary analysis indicates that this isolate, BAK1, could be the first reported strain belonging to a new HCV-7b subtype. This new subtype has been incorrectly identified as genotype 2 by the Versant HCV Genotype 2.0 assay (LiPA). The requirement of three independent isolates has been filled, and a new subtype can be assigned. More examples of HCV-7 are required to better understand its origin, its pathogenicity and its relationship with genotype 2.