High Quality Independent From a Donor: Human Amniotic Fluid Derived Stem Cells—A Practical Analysis Based on 165 Clinical Cases. Issue 1 (28th June 2016)

• Record Type: Journal Article
• Authors: Bajek, Anna; Olkowska, Joanna; Walentowicz‐Sadłecka, Małgorzata; Walentowicz, Paweł; Sadłecki, Paweł; Grabiec, Marek; Bodnar, Magdalena; Marszałek, Andrzej; Dębski, Robert; Porowińska, Dorota; Czarnecka, Joanna; Kaźmierski, Łukasz; Drewa, Tomasz
• Is Part Of: Journal of cellular biochemistry. Volume 118:Issue 1(2017)
• Page Start: 116
• Page End: 126
• Publication Date: 2016-06-28
• Subjects: AMNIOTIC FLUID -- BIOLOGICAL EVALUATION -- STEM CELLS; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.25618
• Languages: English
• ISSNs: 0730-2312

Abstract

ABSTRACT: The aim of the study was to extend the potential use of human stem cells isolated from amniotic fluid in medical applications by confirming their high homogeneity and quality. Amniotic fluid samples were collected during amniocentesis from 165 women during pregnancy. The proliferation rate, clonogenicity, karyotype, aging process, pluripotent cell markers, expression of surface markers, and the potential to differentiate into adipose, bone and cartilage cells of hAFSCs were analyzed. Obtained results revealed that mesenchymal stem cells could be derived successfully from amniotic fluid, which exhibit properties comparable with MSCs of other origins. It is the first study, in which such a large group of patients was involved. Comprehensive statistical and biological analysis were conducted some of which clearly being innovative in relation to human amniotic fluid‐derived stem cells. J. Cell. Biochem. 118: 116–126, 2017. © 2016 Wiley Periodicals, Inc. Abstract : Biological characteristic of hAFSCs suggests they are ideal for wide use in regenerative medicine. This paper is a step forward standardization the transplantation of hAFSCs, either in differentiated or undifferentiated manner, for various clinical applications.