Glutamate release inhibitor, Riluzole, inhibited proliferation of human hepatocellular carcinoma cells by elevated ROS production. Issue 2 (28th November 2016)

• Record Type: Journal Article
• Authors: Seol, Hyang Sook; Lee, Sang Eun; Song, Joon Seon; Lee, Hye Yong; Park, Sojung; Kim, Inki; Singh, Shree Ram; Chang, Suhwan; Jang, Se Jin
• Is Part Of: Cancer letters. Volume 382:Issue 2(2016)
• Page Start: 157
• Page End: 165
• Publication Date: 2016-11-28
• Subjects: Hepatocellular carcinoma -- Drug repositioning -- Riluzole -- ROS; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2016.08.028
• Languages: English
• ISSNs: 0304-3835

Abstract

Highlights: Riluzole leads to a suppression of cell proliferation in liver cancer cells. Riluzole induced caspase-dependent apoptosis and G2/M cell cycle arrest. Riluzole-treated cells have increased level of inner cellular glutamate. Riluzole-treated cells have decreased the glutathione (GSH) level and increased reactive oxygen species (ROS) production. Riluzole-induced GSH and ROS changes in a Huh7 xenograft cancer modelare shown. Abstract: Liver cancer is one of the common malignancies in many countries and an increasing cause of cancer death. Despite of that, there are few therapeutic options available with inconsistent outcome, raising a need for developing alternative therapeutic options. Through a drug repositioning screening, we identified and investigated the action mechanism of the Riluzole, an amyotrophic lateral sclerosis (ALS) drug, on hepatocellular carcinoma (HCC) therapy. Treatment of the Riluzole leads to a suppression of cell proliferation in liver primary cancer cells and cancer cell lines. In addition, Riluzole induced caspase-dependent apoptosis and G2/M cell cycle arrest in SNU449 and Huh7 cell lines. In a line with the known function of glutamate release inhibitor, we found Riluzole-treated cells have increased the level of inner cellular glutamate that in turn decrease the glutathione (GSH) level and finally augment the reactive oxygen species (ROS) production. We confirm this finding in vivo by showing the Riluzole-induced GSH and ROS changes in a Huh7 xenograft cancer model. Altogether, these data suggest the anti-cancer effect of Riluzole on hepatocellular carcinoma and the suppression of glutamate signaling might be a new target pathway for HCC therapy.