Long non-coding RNA LINC00161 sensitises osteosarcoma cells to cisplatin-induced apoptosis by regulating the miR-645-IFIT2 axis. Issue 2 (28th November 2016)

• Record Type: Journal Article
• Authors: Wang, Yuan; Zhang, Li; Zheng, Xifu; Zhong, Weiliang; Tian, Xiliang; Yin, Baosheng; Tian, Kang; Zhang, Weiguo
• Is Part Of: Cancer letters. Volume 382:Issue 2(2016)
• Page Start: 137
• Page End: 146
• Publication Date: 2016-11-28
• Subjects: Long non-coding RNA -- Chemoresistance -- Apoptosis -- LINC00161 -- IFIT2; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2016.08.024
• Languages: English
• ISSNs: 0304-3835

Abstract

Highlights: LINC00161 was induced by cisplatin in osteosarcoma cells. Elevated LINC00161 enhanced cisplatin-induced apoptosis and reduced chemoresistance. LINC00161 acted as a miR-645 sponge and inhibited its activity. LINC00161 upregulated IFIT2 expression via miR-645 LINC00161 sensitised osteosarcoma cell to cisplatin-induced apoptosis through regulation of the miR-645-IFIT2 axis. Abstract: Chemotherapeutic insensitivity remains a major obstacle to osteosarcoma treatment. Recently, increasing evidence has suggested that long non-coding RNAs (lncRNAs) play an essential role in tumourigenesis. However, the potential biological roles and regulatory mechanisms of novel lncRNAs in response to cisplatin treatment are poorly understood. Here, we found that lncRNA LINC00161 was induced by cisplatin in osteosarcoma cells. Elevated LINC00161 increased cisplatin-induced apoptosis and reversed the cisplatin-resistant phenotype of osteosarcoma cells by upregulating IFIT2 . Further mechanistic studies revealed that LINC00161 could sponge endogenous miR-645 and inhibit its activity leading to IFIT2 increase. In addition, we identified that LINC00161 enhanced cisplatin-induced apoptosis through regulation of the miR-645 - IFIT2 pathway. Thus, these findings demonstrate that LINC00161 is an essential regulator in cisplatin-induced apoptosis, and the LINC00161 - miR-645 - IFIT2 signalling axis plays an important role in reducing osteosarcoma chemoresistance.