Raman spectroscopy in experimental oral carcinogenesis: investigation of abnormal changes in control tissues. (28th June 2016)

• Record Type: Journal Article
• Authors: Kumar, Piyush; Bhattacharjee, Tanmoy; Pandey, Manishkumar; Hole, Arti; Ingle, Arvind; Murali Krishna, C.
• Is Part Of: Journal of Raman spectroscopy. Volume 47:Number 11(2016)
• Page Start: 1318
• Page End: 1326
• Publication Date: 2016-06-28
• Subjects: Raman spectroscopy -- hamster buccal pouch -- experimental oral carcinogenesis -- chronic irritation -- DMBA; Raman spectroscopy -- Periodicals; 535.846
• DOI: 10.1002/jrs.4977
• Languages: English
• ISSNs: 0377-0486

Abstract

Abstract : Oral cancer is a major cause of mortality in South Asian men owing to rampant tobacco abuse. Cancers are also reported in non‐tobacco habitués, especially women, attributed to chronic irritations from irregular/sharp teeth, improper fillings, and poorly fit dentures. Conventional screening approaches are shown to be effective for high‐risk groups (tobacco/alcohol habitués). Raman spectroscopy (RS) is being extensively explored as an alternate/adjunct tool for diagnosis and management of oral cancers. In a previous Raman study on sequential oral carcinogenesis using hamster buccal pouch model, misclassifications between spectra from control and carcinogen [7, 12‐dimethylbenz( a )anthracene (DMBA)]‐treated tissues were observed. Histopathology of some control tissues suggested pathological changes, attributable to repeated forceps‐induced irritations/trauma during animal handling. To explore these changes, in the present study, we recorded spectra from three different types of controls – vehicle control ( n = 45), vehicle contralateral ( n = 45), and DMBA contralateral ( n = 70) – exposed to varying degree of forceps handling, along with DMBA‐treated pouches ( n = 70) using a 14‐week carcinogenesis protocol. Spectra certified on the basis of histopathology and abnormal cell proliferation (cyclin D1 expression) were used to build models that were evaluated by independent test spectra from an exclusive set of DMBA‐treated and control animals. Many DMBA‐contralateral, vehicle‐control, and vehicle‐contralateral spectra were identified as higher pathologies, which subsequently corroborated with histopathology/cyclin D1 expression. Repeated forceps‐mediated injuries/irritations, during painting and animal handling, may elicit inflammatory responses, leading to neoplasm. The findings of the study suggest that RS could identify micro‐changes. Further, RS‐based in vivo imaging can serve as a promising label‐free tool for screening even in the non‐habitué population where conventional screening is shown to be not effective. Copyright © 2016 John Wiley & Sons, Ltd. Abstract : Raman spectra acquired from controls – exposed to varying degree of forceps handling (vehicle control, vehicle contralateral, and carcinogen contralateral) – were evaluated against histopathology/immunohistochemistry‐based models. Findings suggest that RS can identify micro‐changes, including squamous cell carcinoma, probably owing to repeated irritation. Chronic irritation is a known etiological agent for oral cancers. Thus, the present study further demonstrated the efficacy of Raman spectroscopy as a potential tool for screening, in both high‐risk and non‐tobacco habitués groups, vis‐à‐vis conventional screening, which is shown to be more effective in high‐risk population.