Enzymatic activity of cell-free extracts from Burkholderia oxyphila OX-01 bio-converts (+)-catechin and (−)-epicatechin to (+)-taxifolin. Issue 12 (1st December 2016)

• Record Type: Journal Article
• Authors: Otsuka, Yuichiro; Matsuda, Motoki; Sonoki, Tomonori; Sato-Izawa, Kanna; Goodell, Barry; Jelison, Jody; Navarro, Ronald R.; Murata, Hitoshi; Nakamura, Masaya
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 12(2016)
• Page Start: 2473
• Page End: 2479
• Publication Date: 2016-12-01
• Subjects: burkholderia -- (+)-catechin -- (+)-taxifolin -- (−)-epicatechin -- enzymatic reaction; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2016.1220822
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract : This study characterized the enzymatic ability of a cell-free extract from an acidophilic (+)-catechin degrader Burkholderia oxyphila (OX-01). The crude OX-01 extracts were able to transform (+)-catechin and (− )-epicatechin into (+)-taxifolin via a leucocyanidin intermediate in a two-step oxidation. Enzymatic oxidation at the C-4 position was carried out anaerobically using H2 O as an oxygen donor. The C-4 oxidation occurred only in the presence of the 2R-catechin stereoisomer, with the C-3 stereoisomer not affecting the reaction. These results suggest that the OX-01 may have evolved to target both (+)-catechin and (− )-epicatechin, which are major structural units in plants. Graphical abstract: Proposed anaerobic oxidation pathway of (+)-catechin and (−)-epicatechin by Burkholderia oxyphila OX-01.